表面增强拉曼散射（SERS）细胞计数法。
Surface-enhanced Raman scattering (SERS) cytometry.
作者信息
Nolan John P, Sebba David S
机构信息
La Jolla Bioengineering Institute, La Jolla, California, USA.
出版信息
Methods Cell Biol. 2011;102:515-32. doi: 10.1016/B978-0-12-374912-3.00020-1.
Abstract
Significant advances have been made in the preparation and applications of surface-enhanced Raman scattering (SERS)-active materials for biomolecular analysis. Bright signals, photostability, and narrow spectral features of SERS-active materials offer attractive advantages for cytometric analyses. However, SERS cytometry is still in an early stage of development, and advances in both instrumentation and reagents will be necessary to realize its full potential. In this chapter, we discuss the challenges of expanding the numbers of fluorescent labels that can be measured in cytometry, and introduce SERS tags with extremely narrow spectral peaks as an approach to make more efficient use of the optical spectrum and increase the number of parameters in cytometry.
摘要
在用于生物分子分析的表面增强拉曼散射（SERS）活性材料的制备和应用方面已经取得了重大进展。SERS活性材料的明亮信号、光稳定性和窄光谱特征为细胞分析提供了诱人的优势。然而，SERS细胞术仍处于发展初期，要充分发挥其潜力，仪器和试剂方面都需要取得进展。在本章中，我们讨论了扩大细胞术中可测量的荧光标记数量所面临的挑战，并介绍了具有极窄光谱峰的SERS标签，作为一种更有效地利用光谱并增加细胞术中参数数量的方法。
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