D'Ambola J B, Aeberhard E E, Trang N, Gaffar S, Barrett C T, Sherman M P
University of California, Department of Pediatrics-Division of Neonatology, Los Angeles 90024-1752.
J Nutr. 1991 Aug;121(8):1262-9. doi: 10.1093/jn/121.8.1262.
Intrapulmonary bacterial clearance, lung inflammatory cell recruitment and macrophage superoxide generating capacity were studied in newborn rabbits nursed by their mother and given a supplement of either high [5 g/(kg.d)] or low [0.22 g/(kg.d)] doses of fish oil, safflower oil or saline for 7 d after birth. The high dose fish and safflower oil regimens diminished lung clearance of inspired Staphylococcus aureus by approximately 50% compared with the saline controls, but they did not alter lung neutrophil recruitment or alveolar macrophage bacterial phagocytosis. Only high dose fish oil decreased macrophage superoxide anion generation (by 30%). With high dose fish or safflower oil supplementation, the fatty acid content of lung parenchyma, bronchoalveolar lavage effluent and alveolar macrophages increased significantly. Low dose supplementation did not have this effect. We conclude that pharmacologic dietary (n-6) and (n-3) fatty acid supplementation impairs the ability of the neonatal rabbit lung to kill intrapulmonary S. aureus. It has been proposed that human infant formula be supplemented with fatty acids either to alleviate dietary deficiencies or to treat pulmonary inflammatory disorders. Based on our findings, the effects of such supplementation should be monitored relative to the possible heightened risk of bacterial infection.
对由母兔哺育的新生兔进行了研究，观察其肺内细菌清除、肺炎症细胞募集及巨噬细胞超氧化物生成能力。新生兔出生后7天，分别给予高剂量[5克/（千克·天）]或低剂量[0.22克/（千克·天）]的鱼油、红花油或生理盐水补充剂。与生理盐水对照组相比，高剂量鱼油和红花油方案使吸入的金黄色葡萄球菌的肺清除率降低了约50%，但未改变肺中性粒细胞募集或肺泡巨噬细胞细菌吞噬作用。只有高剂量鱼油降低了巨噬细胞超氧阴离子的生成（降低30%）。补充高剂量鱼油或红花油后，肺实质、支气管肺泡灌洗流出液和肺泡巨噬细胞的脂肪酸含量显著增加。低剂量补充则无此作用。我们得出结论，药理学剂量的膳食（n-6）和（n-3）脂肪酸补充会损害新生兔肺杀灭肺内金黄色葡萄球菌的能力。有人提出在人类婴儿配方奶粉中添加脂肪酸以缓解膳食缺乏或治疗肺部炎症性疾病。基于我们的研究结果，相对于可能增加的细菌感染风险，应监测这种补充的效果。
