Caban A, Oczkowicz G, Abdel-Samad O, Cierpka L
Department of General, Vascular and Transplant Surgery, Medical University of Silesia, Francuska 20/24, 40-027 Katowice, Poland.
Transplant Proc. 2006 Jan-Feb;38(1):196-8. doi: 10.1016/j.transproceed.2005.12.032.
The aim of this study was to assess the influence of ischemic preconditioning (IPC) on parenchymal liver blood flow during the early phase of reperfusion after 60 minutes of ischemia, additionally modified by adding N-nitro-L-arginine methyl ester (L-NAME). Our research involved 4 groups of rats (10 animals in each group), which underwent liver ischemia and 24 hours of reperfusion. Group I, ischemia/reperfusion (IR) was performed; group II, IPC, 10 minutes of ischemia and 10 minutes of reperfusion, and IR after that; group III, L-NAME (10 mg/kg intravenous [iv]), 10 minutes before IR; and group IV, L-NAME before IPC + IR. Activity of APAT, ALAT, GGTP, and FA was marked in serum in 90 minutes and 24 hours of reperfusion. In the liver biopsies at 24 hours of reperfusion, we analyzed reaction on adenosine-3-phosphatase stimulated by Mg++ and performed histological examination. The parenchymal perfusion was measured using a laser-doppler blood flowmeter (model PeriFlux System5000, Perimed Inc., United Kingdom). IPC during reperfusion led to minor injuries of the organ, with statistically significant normalization of enzymes compared with group 1, and a better reaction to the adenosine-3-phosphatase IPC produced faster and full return of perfusion to the 68.3 value at 24 hours (59.1 in the 60 minutes). In groups III and IV at 60 minutes, the perfusion was not statistically different from that in group 1. IPC causes full and faster blood return in the early phase of reperfusion and minor injury of liver parenchyma and liver sinus. The protective effect observed, especially in the first 60 minutes of reperfusion, was limited by L-NAME and was influenced by the action of nitric oxide.
本研究的目的是评估缺血预处理（IPC）对缺血60分钟后再灌注早期肝实质血流的影响，另外通过添加N-硝基-L-精氨酸甲酯（L-NAME）进行了改良。我们的研究涉及4组大鼠（每组10只动物），它们经历了肝脏缺血和24小时再灌注。第一组，进行缺血/再灌注（IR）；第二组，IPC，缺血10分钟和再灌注10分钟，然后进行IR；第三组，在IR前10分钟静脉注射（iv）L-NAME（10mg/kg）；第四组，在IPC+IR前注射L-NAME。在再灌注90分钟和24小时时测定血清中APAT、ALAT、GGTP和FA的活性。在再灌注24小时时进行肝脏活检，我们分析了Mg++刺激的腺苷三磷酸酶反应并进行了组织学检查。使用激光多普勒血流仪（PeriFlux System5000型号，Perimed公司，英国）测量实质灌注。再灌注期间的IPC导致器官轻微损伤，与第一组相比，酶的统计学显著正常化，并且对腺苷三磷酸酶的反应更好，IPC使灌注在24小时时更快且完全恢复到68.3的值（60分钟时为59.1）。在第三组和第四组60分钟时，灌注与第一组相比无统计学差异。IPC在再灌注早期导致血流完全且更快恢复，以及肝实质和肝窦轻微损伤。观察到的保护作用，特别是在再灌注的前60分钟，受到L-NAME的限制，并受一氧化氮作用的影响。
