Nieuwenhuijs Vincent B, de Bruijn Menno T, Schiesser Marc, Morphett Arthur, Padbury Robert T A, Barritt Greg J
The HPB and Liver Transplant Unit, Flinders Medical Centre and School of Medicine, Flinders University, GPO Box 2100, Adelaide, South Australia, 5001, Australia.
Dig Dis Sci. 2007 Nov;52(11):3029-37. doi: 10.1007/s10620-006-9501-x. Epub 2007 Jul 31.
Ischemia and reperfusion (IR) injury of the liver is associated with impaired bile secretion, but the effects of ischemic preconditioning (IPC) and intermittent ischemia (INT) on bile flow are unknown. A rat model of segmental (60%-70%) hepatic ischemia and reperfusion was employed to test the effects of IPC and INT on bile flow. Continuous clamping for 45 min (CC) substantially reduced bile flow, and this did not recover after 60 min of reperfusion. IPC and INT caused a significant recovery of bile flow. The elevation in plasma liver marker enzymes induced by CC was not reduced by IPC and INT. Light microscopy showed mild hepatocyte damage in all groups. In the CC group, the amount of F-actin localized around the bile canaliculi in the ischemic lobes was less than that in the nonischemic lobes, but this difference was not observed in the IPC and INT groups. It is concluded that IPC and INT substantially alleviate the decrease in bile flow induced by ischemia. Bile flow may be useful in the assessment of IR injury.
肝脏缺血再灌注（IR）损伤与胆汁分泌受损有关，但缺血预处理（IPC）和间歇性缺血（INT）对胆汁流动的影响尚不清楚。采用大鼠节段性（60%-70%）肝脏缺血再灌注模型来测试IPC和INT对胆汁流动的影响。持续夹闭45分钟（CC）可显著降低胆汁流动，且在再灌注60分钟后仍未恢复。IPC和INT可使胆汁流动显著恢复。CC诱导的血浆肝脏标志物酶升高并未因IPC和INT而降低。光学显微镜检查显示所有组均有轻度肝细胞损伤。在CC组中，缺血叶胆小管周围的F-肌动蛋白量少于非缺血叶，但在IPC和INT组中未观察到这种差异。得出的结论是，IPC和INT可显著减轻缺血诱导的胆汁流动减少。胆汁流动可能有助于评估IR损伤。
