Cibull T L, Jones T D, Li L, Eble J N, Ann Baldridge L, Malott S R, Luo Y, Cheng L
Department of Pathology, Indiana University School of Medicine, Indianapolis, USA.
J Clin Pathol. 2006 Mar;59(3):285-8. doi: 10.1136/jcp.2005.027672.
Pim-1 is a serine/threonine kinase that has been shown to play an integral role in the development of a number of human cancers, such as haematolymphoid malignancies. Recently, evidence has shown Pim-1 to be important in prostatic carcinogenesis. In order to further our understanding of its role in prostate cancer, we investigated Pim-1 expression in normal, premalignant, and malignant prostate tissue.
Using immunohistochemistry, Pim-1 expression was analysed in prostate tissue from 120 radical prostatectomy specimens. In each case, Pim-1 staining was evaluated in benign prostatic epithelium, high grade prostatic intraepithelial neoplasia (PIN), and prostatic adenocarcinoma. The number of positively staining cells was estimated, and the intensity of staining was scored on a scale of 0 to 3+.
Pim-1 immunoreactivity was identified in 120 cases (100%) of adenocarcinoma, 120 cases (100%) of high grade PIN, and 62 cases (52%) of benign glands. The number of cells staining in benign epithelium (mean 34%) was much lower than that in high grade PIN (mean 80%; p<0.0001) or adenocarcinoma (mean, 84%; p<0.0001). There was no significant difference between high grade PIN and adenocarcinoma in the percentage of cells staining positively for Pim-1 (p = 0.34). The staining intensity for Pim-1 was significantly lower in benign prostatic epithelium than in PIN and adenocarcinoma (p<0.001). There was no statistically significant correlation between the level of Pim-1 expression and Gleason score, patient age, tumour stage, lymph node metastasis, perineural invasion, vascular invasion, surgical margin status, extraprostatic extension, or seminal vesicle invasion.
Pim-1 expression is elevated in PIN and prostatic adenocarcinoma compared with benign prostatic epithelium. This finding suggests that upregulation of Pim-1 may play a role in prostatic neoplasia.
Pim-1是一种丝氨酸/苏氨酸激酶，已被证明在多种人类癌症（如血液淋巴系统恶性肿瘤）的发展中发挥着不可或缺的作用。最近，有证据表明Pim-1在前列腺癌发生过程中很重要。为了进一步了解其在前列腺癌中的作用，我们研究了Pim-1在正常、癌前和恶性前列腺组织中的表达情况。
采用免疫组织化学方法，对120例前列腺癌根治术标本的前列腺组织进行Pim-1表达分析。在每例标本中，评估良性前列腺上皮、高级别前列腺上皮内瘤变（PIN）和前列腺腺癌中的Pim-1染色情况。估计阳性染色细胞的数量，并将染色强度按0至3+进行评分。
在120例（100%）腺癌、120例（100%）高级别PIN和62例（52%）良性腺体中均检测到Pim-1免疫反应性。良性上皮中染色的细胞数量（平均34%）远低于高级别PIN（平均80%；p<0.0001）或腺癌（平均84%；p<0.0001）。高级别PIN和腺癌中Pim-1阳性染色细胞的百分比无显著差异（p = 0.34）。良性前列腺上皮中Pim-1的染色强度明显低于PIN和腺癌（p<0.001）。Pim-1表达水平与Gleason评分、患者年龄、肿瘤分期、淋巴结转移、神经周围浸润、血管浸润、手术切缘状态、前列腺外扩展或精囊侵犯之间无统计学显著相关性。
与良性前列腺上皮相比，PIN和前列腺腺癌中Pim-1表达升高。这一发现表明Pim-1的上调可能在前列腺肿瘤形成中起作用。
