Shkurnikov M Yu, Nersisyan S A, Osepyan A Sh, Maltseva D V, Knyazev E N
Hertsen Moscow Oncology Research Center, Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia.
Faculty of Biology and Biotechnology, National Research University Higher School of Economics, Moscow, Russia.
Dokl Biochem Biophys. 2020 Jul;493(1):208-210. doi: 10.1134/S1607672920040122. Epub 2020 Sep 7.
Human colorectal adenocarcinoma cell line Caco-2 is often used as a model of healthy intestinal epithelium, in particular, in miRNA studies. The work of the enzymes Drosha and Dicer is an integral part of the process of miRNA formation. Inaccuracies in the work of these enzymes lead to a change in the nucleotide sequences of miRNAs with the formation of new isoforms, which, in turn, can change intracellular regulatory mechanisms. In the framework of this study, it was shown that the quantitative estimates of inaccuracies in Drosha and Dicer activity significantly differ between the specimens of normal colon tissue and malignant colorectal tumors.
人结肠腺癌细胞系Caco-2常被用作健康肠上皮的模型，尤其是在miRNA研究中。Drosha和Dicer酶的作用是miRNA形成过程中不可或缺的一部分。这些酶作用的不准确会导致miRNA核苷酸序列发生变化并形成新的异构体，进而可能改变细胞内的调节机制。在本研究框架内，结果表明，正常结肠组织标本和恶性结肠直肠肿瘤标本中Drosha和Dicer活性不准确的定量估计存在显著差异。
