Kasashima Katsumi, Nakamura Yoshikazu, Kozu Tomoko
Research Institute for Clinical Oncology, Saitama Cancer Center, Ina, Saitama 362-0806, Japan.
Biochem Biophys Res Commun. 2004 Sep 17;322(2):403-10. doi: 10.1016/j.bbrc.2004.07.130.
MicroRNAs (miRNAs) are highly conserved small non-coding RNAs that regulate gene expression through translational repression by base-pairing with partially complementary mRNAs. The expression of a set of miRNAs is known to be regulated developmentally and spatially, and is involved in differentiation or cell proliferation in several organisms. However, the expression profiles of human miRNAs during cell differentiation remain largely unknown. In an effort to expand our knowledge of human miRNAs, we investigated miRNAs during 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced differentiation of human leukemia cells (HL-60) into monocyte/macrophage-like cells. Several hundred RNAs ranging from 18 to 26 nucleotides were isolated from HL-60 cells with or without TPA-induction, and subsequently characterized by sequencing, database searching, and expression profiling. By removing non-miRNA sequences, we found three novel and 38 known miRNAs expressed in HL-60 cells. These miRNAs could be further classified into subsets of miRNAs that responded differently following TPA induction, either being up-regulated or down-regulated, suggesting the importance of regulated gene expression via miRNAs in the differentiation of HL-60 cells.
微小RNA（miRNA）是高度保守的小非编码RNA，通过与部分互补的mRNA碱基配对来调控基因表达，从而实现翻译抑制。已知一组miRNA的表达受到发育和空间上的调控，并参与多种生物体的分化或细胞增殖过程。然而，人类miRNA在细胞分化过程中的表达谱仍很大程度上未知。为了扩展我们对人类miRNA的认识，我们研究了在12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）诱导人白血病细胞（HL - 60）分化为单核细胞/巨噬细胞样细胞过程中的miRNA。从有或无TPA诱导的HL - 60细胞中分离出数百个长度在18至26个核苷酸之间的RNA，随后通过测序、数据库搜索和表达谱分析对其进行表征。通过去除非miRNA序列，我们在HL - 60细胞中发现了3个新的和38个已知的miRNA。这些miRNA可进一步分为在TPA诱导后有不同反应的miRNA子集，有的上调，有的下调，这表明通过miRNA调控基因表达在HL - 60细胞分化中具有重要作用。
