鲑鱼疱疹病毒（马苏大麻哈鱼病毒）DNA针对三种限制性内切酶BamHI、EcoRI和XhoI的部分限制性图谱。

Partial restriction map of salmonid herpesvirus (Oncorhynchus masou virus) DNA for three restriction enzymes BamHI, EcoRI and XhoI.

作者信息

Guo D F, Kodama H, Onuma M

机构信息

Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.

出版信息

J Vet Med Sci. 1991 Apr;53(2):213-8. doi: 10.1292/jvms.53.213.

DOI:10.1292/jvms.53.213

PMID:1650600

Abstract

Cleavage of Oncorhynchus masou virus (OMV) DNA with restriction endonucleases BamHI, EcoRI, and XhoI resulted in 28, 26 and 17 fragments, respectively. Based on the molecular weights of digested fragments and those molar ratio. OMV DNA showed the molecular weight of about 100 x 10(6). Twenty out of 28 BamHI fragments of OMV DNA were successfully cloned into pBR322 vector. Restriction map of OMV DNA was constructed by blotting hybridization and double-digestion. The data suggested that terminal repeat of the end fragments of OMV DNA molecule was not existence.

摘要

用限制性内切酶BamHI、EcoRI和XhoI切割马苏大麻哈鱼病毒（OMV）DNA，分别产生了28、26和17个片段。根据消化片段的分子量和摩尔比，OMV DNA的分子量约为100×10⁶。OMV DNA的28个BamHI片段中有20个成功克隆到pBR322载体中。通过印迹杂交和双酶切构建了OMV DNA的限制图谱。数据表明，OMV DNA分子末端片段不存在末端重复序列。