Lin J A
Department of Veterinary Medicine, College of Agriculture, National Chung Hsing University, Taichung, Taiwan, ROC.
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1991 Nov;24(4):321-30.
Herpesvirus salmonis (H. salmonis) and seven strains of Oncorhynchus masou virus (OMV) were analyzed for their genomes with restriction endonucleases HindIII and PstI. Restriction profiles of H. salmonis were different from those of OMV strains. Restriction patterns of OMV strain DNAs were divided into four subtypes with HindIII. With PstI digestion, DNA restriction patterns of those strains were divided into five subtypes. Obvious difference in restriction patterns was not observed among tumor tissue-derived and coelomic fluids-derived strains. By using 32P-labelled DNA of standard OMV (strain 00-7812) as a probe, H. salmonis DNA was not hybridized, but most of the fragments of OMV strain DNAs were hybridized.
利用限制性内切酶HindIII和PstI对鲑疱疹病毒(H. salmonis)和七种马苏大麻哈鱼病毒(OMV)毒株的基因组进行了分析。鲑疱疹病毒的限制性图谱与OMV毒株的不同。用HindIII酶切时,OMV毒株DNA的限制性图谱可分为四个亚型。用PstI酶切时,这些毒株的DNA限制性图谱可分为五个亚型。肿瘤组织来源的毒株和体腔液来源的毒株之间未观察到明显的限制性图谱差异。以标准OMV(00 - 7812毒株)的32P标记DNA为探针,鲑疱疹病毒DNA未杂交,但OMV毒株DNA的大部分片段都发生了杂交。