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1型马疱疹病毒基因组分析:限制性内切酶EcoRI、BglII和BamHI切割位点的排列

Analysis of the genome of equine herpesvirus type 1: arrangement of cleavage sites for restriction endonucleases EcoRI, BglII and BamHI.

作者信息

Whalley J M, Robertson G R, Davison A J

出版信息

J Gen Virol. 1981 Dec;57(Pt 2):307-23. doi: 10.1099/0022-1317-57-2-307.

DOI:10.1099/0022-1317-57-2-307
PMID:6275028
Abstract

The genome of an Australian isolate of equine herpesvirus type 1 (equine abortion virus) has been analysed using the restriction endonucleases EcoRI, BglII and BamHI, and a physical map constructed. Terminal fragments were identified by exonuclease treatments, and linkage of fragments was deduced by a combination of single- and double-digest experiments and cross-blot hybridizations. The genome has a mol. wt. of 100 x 10(6) and is comprised of a short unique region bounded by repetitive sequences, which is present in both orientations in approximately equal amounts in the DNA population, and a long unique region existing in only one orientation.

摘要

利用限制性内切酶EcoRI、BglII和BamHI对一株澳大利亚1型马疱疹病毒(马流产病毒)的基因组进行了分析,并构建了物理图谱。通过核酸外切酶处理鉴定末端片段,并通过单酶切和双酶切实验以及交叉印迹杂交相结合的方法推断片段的连接情况。该基因组的分子量为100×10⁶,由一个短的单一区域和一个长的单一区域组成,短单一区域由重复序列界定,在DNA群体中以两种方向存在且数量大致相等,长单一区域仅以一种方向存在。

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