Sekiguchi Jun-Ichiro, Fujino Tomoko, Araake Minako, Toyota Emiko, Kudo Koichiro, Saruta Katsutoshi, Yoshikura Hiroshi, Kuratsuji Tadatoshi, Kirikae Teruo
International Medical Center of Japan, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan.
J Infect Chemother. 2006 Feb;12(1):47-50. doi: 10.1007/s10156-005-0417-8.
To assess whether the occurrence of rifampicin (RFP) resistance in methicillin-resistant Staphylococcus aureus (MRSA) is related to treatment of tuberculosis, we determined the RFP susceptibility of MRSA isolates obtained from tuberculosis patients and screened for mutation(s) in the rpoB gene of these isolates. The MICs of RFP for 84 MRSA isolates obtained from two hospitals in Japan were determined. DNA was sequenced in the region 1318-1602 nucleotides (nt) of the rpoB gene, which includes RFP resistance-determining clusters I (1384-1464 nt, 462-488 amino acids). The majority of MRSA isolates from tuberculosis wards, i.e., 48 of 51 (94%) [33 of 34 in a Tokyo hospital (97%) and 15 of 17 in a Chubu hospital (88%)], were resistant to RFP. Meanwhile, no isolates of 33 from the other wards were resistant to RFP. All RFP-resistant MRSA isolates had a mutation(s), including novel mutation(s) such as Val453-->AEPhe, Asp471-->AEAsn, and Ile527-->AELeu, in rpoB. An emergence of RFP-resistant MRSAs in tuberculosis wards in Japan was strongly suggested.
为评估耐甲氧西林金黄色葡萄球菌（MRSA）中利福平（RFP）耐药性的发生是否与结核病治疗有关，我们测定了从结核病患者分离出的MRSA菌株对RFP的敏感性，并筛查了这些菌株rpoB基因中的突变。测定了从日本两家医院获得的84株MRSA菌株对RFP的最低抑菌浓度（MIC）。对rpoB基因1318 - 1602核苷酸（nt）区域进行DNA测序，该区域包括RFP耐药决定簇I（1384 - 1464 nt，462 - 488个氨基酸）。结核病病房的大多数MRSA菌株，即51株中的48株（94%）[东京一家医院的34株中有33株（97%），中部一家医院的17株中有15株（88%）]对RFP耐药。同时，其他病房的33株菌株均无RFP耐药。所有耐RFP的MRSA菌株在rpoB基因中都有一个或多个突变，包括新的突变，如Val453→AEPhe、Asp471→AEAsn和Ile527→AELeu。强烈提示日本结核病病房出现了耐RFP的MRSA。
