丁香假单胞菌中β-内酰胺抗生素烟草毒素的生物合成基因簇。

The biosynthetic gene cluster for the beta-lactam antibiotic tabtoxin in Pseudomonas syringae.

作者信息

Kinscherf Thomas G, Willis David K

机构信息

USDA/ARS Vegetable Crops Research Unit and Department of Plant Pathology, University of Wisconsin - Madison, Madison, WI 53706, USA.

出版信息

J Antibiot (Tokyo). 2005 Dec;58(12):817-21. doi: 10.1038/ja.2005.109.

DOI:10.1038/ja.2005.109

PMID:16506699

Abstract

DNA sequence analysis revealed that the biosynthetic genes of the unusual beta-lactam antibiotic tabtoxin reside at the att site adjacent to the lysC tRNA gene in Pseudomonas syringae BR2. ORFs encoded within the region included ones with similarity to beta-lactam synthase and clavaminic acid synthase, as well as amino acid synthesis enzymes. Novel ORFs were present in a portion of the biosynthetic region associated with a toxin hypersensitivity phenotype. Tabtoxin resistance was associated with a fragment containing a major facilitator superfamily (MFS) transporter gene.

摘要

DNA序列分析表明，异常β-内酰胺抗生素烟草毒素的生物合成基因位于丁香假单胞菌BR2中与lysC tRNA基因相邻的附着位点。该区域内编码的开放阅读框包括与β-内酰胺合酶和棒酸合酶相似的基因，以及氨基酸合成酶。在与毒素超敏表型相关的生物合成区域的一部分中存在新的开放阅读框。烟草毒素抗性与一个包含主要易化子超家族（MFS）转运蛋白基因的片段相关。