Labella F, Dular R, Queen G, Vivian S
Endocrinology. 1975 Jun;96(6):1559-65. doi: 10.1210/endo-96-6-1559.
Release of hormones from bovine anterior pituitary tissue in vitro was inveresly related to the osmolarity of the incubation medium. Addition of each of several ionic or non-ionic solutes to Krebs-Ringer-bicarbonate (KRB) or to beef serum inhibited hormone release. If the value of 286 mOsm/liter of KRB is considered as 100%, the osmolarity of the medium was altered from -15% to +10% of control. Over this range, an increase in osmolarity of 10% resulted in the following percentage inhibition: ACTH, 47; PRL, 43; TSH, 36; LH, 23; GH, 18; maximal percentage inhibition over this range was as follows: ACTH, 80; PRL, 60; TSH, 60; LH, 45; GH, 40. The inverse relationship between extracellular oxmolarity and secretion of ACTH and PRL would appear to be appropriate in view of the salt and water retaining actions of these hormones. The sensitivity of the response to osmotic changes suggests a possible role of body osmolarity in the regulation of adenohypophysial secretion.
牛垂体前叶组织在体外释放激素的量与孵育介质的渗透压呈负相关。向克雷布斯 - 林格 - 碳酸氢盐（KRB）或牛血清中添加几种离子或非离子溶质中的每一种都会抑制激素释放。如果将KRB的286毫渗摩尔/升的值视为100%，则培养基的渗透压在对照的 - 15%至 + 10%之间变化。在此范围内，渗透压增加10%会导致以下百分比抑制：促肾上腺皮质激素（ACTH），47；催乳素（PRL），43；促甲状腺激素（TSH），36；促黄体生成素（LH），23；生长激素（GH），18；此范围内的最大百分比抑制如下：ACTH，80；PRL，60；TSH，60；LH，45；GH，40。鉴于这些激素的保盐和保水作用，细胞外渗透压与ACTH和PRL分泌之间的负相关似乎是合理的。对渗透压变化的反应敏感性表明身体渗透压在腺垂体分泌调节中可能起作用。
