Lopez Ignacio, Estepa Jose C, Mendoza Francisco J, Mayer-Valor Rafael, Aguilera-Tejero Escolastico
Department of Medicina y Cirugia Animal, Universidad de Cordoba, Campus Universitario Rabanales, Ctra Madrid-Cadiz km 396, 14014 Cordoba, Spain.
Am J Vet Res. 2006 Mar;67(3):463-6. doi: 10.2460/ajvr.67.3.463.
To establish reference values for protein-bound, ionized, and weak-acid complexed fractions of calcium and magnesium in equine serum and determine stability of ionized calcium (iCa) and ionized magnesium (iMg) in serum samples kept under various storage conditions.
28 clinically normal horses.
Total calcium (tCa) and magnesium (tMg) in equine serum were fractionated by use of a micropartition system that allows separation of protein-bound calcium (pCa) and magnesium (pMg) and ultrafiltrable calcium (microCa) and magnesium (microMg) fractions. Serum concentrations of iCa and iMg were measured in the ultrafiltrate by use of selective electrodes. Serum concentration of complexed calcium (cCa) or magnesium (cMg) was calculated by subtracting iCa or iMg from microCa or microMg, respectively.
Mean +/-SE serum tCa concentration was 3.26 +/- 0.06 mmol/L. Calcium fractions were as follows: pCa, 1.55 +/- 0.03 mmol/L (47.4 +/- 0.9%); iCa, 1.58 +/- 0.03 mmol/L (48.5 +/- 0.7%); and cCa, 0.13 +/- 0.02 mmol/L (4.1 +/- 0.9%). Serum tMg concentration was 0.99 +/- 0.04 mmol/L. Magnesium fractions were as follows: pMg, 0.33 +/- 0.04 mmol/L (33.3 +/- 4.2%); iMg, 0.57 +/- 0.02 mmol/L (57.6 +/- 1.7%); and cMg, 0.09 +/- 0.02 mmol/L (9.1 +/- 1.9%). Refrigeration (4 degrees C) did not affect iCa values, whereas iMg declined by 8% after 120 hours. Neither iCa nor iMg was affected by freezing (-20 degrees C).
In equine serum, iMg is less stable than iCa; thus, when serum samples are not going to be analyzed promptly, freezing may be preferable to refrigeration for storage.
建立马血清中钙和镁的蛋白结合、离子化及弱酸络合部分的参考值，并确定在不同储存条件下血清样本中离子化钙（iCa）和离子化镁（iMg）的稳定性。
28匹临床正常的马。
使用微分区系统对马血清中的总钙（tCa）和镁（tMg）进行分离，该系统可分离蛋白结合钙（pCa）和镁（pMg）以及可超滤钙（microCa）和镁（microMg）部分。通过使用选择性电极测量超滤液中iCa和iMg的血清浓度。络合钙（cCa）或镁（cMg）的血清浓度分别通过从microCa或microMg中减去iCa或iMg来计算。
血清tCa平均浓度为3.26±0.06 mmol/L。钙的各部分如下：pCa，1.55±0.03 mmol/L（47.4±0.9%）；iCa，1.58±0.03 mmol/L（48.5±0.7%）；cCa，0.13±0.02 mmol/L（4.1±0.9%）。血清tMg浓度为0.99±0.04 mmol/L。镁的各部分如下：pMg，0.33±0.04 mmol/L（33.3±4.2%）；iMg，0.57±0.02 mmol/L（57.6±1.7%）；cMg，0.09±0.02 mmol/L（9.1±1.9%）。冷藏（4℃）不影响iCa值，而iMg在120小时后下降了8%。iCa和iMg均不受冷冻（-20℃）影响。
在马血清中，iMg比iCa稳定性差；因此，当血清样本不能立即分析时，冷冻储存可能比冷藏更可取。
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