Das S S, Chaudhary R, Khetan D, Shukla J S, Agarwal P, Mishra R B
Transfusion Medicine, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.
Transfus Med. 2005 Jun;15(3):233-6. doi: 10.1111/j.1365-3148.2005.00576.x.
It is well known that citrate induces ionized hypocalcaemia by the chelating effect during plateletpheresis. However, the kinetics of serum magnesium (Mg) ions has not been well documented. We, therefore, evaluated biochemical changes in healthy donors during plateletpheresis procedure. Ten healthy donors underwent plateletpheresis on continuous cell separator (CS3000, Baxter, Round Lake, IL, USA) and 10 on intermittent flow cell separator (MCS 3p, Hemonetics, Braintree, MA, USA). Serum levels of total and ionized calcium (tCa and iCa, respectively) and Mg (tMg and iMg, respectively) were measured before, during and after the procedures. Although, the fall in tCa (from 2.62 +/- 0.12 to 2.36 +/- 0.12 mmol L(-1)) and tMg (from 0.89 +/- 0.01 to 0.79 +/- 0.01 mmol L(-1)) was modest and not significant; drop in iCa (from 1.33 +/- 0.1 to 0.84 +/- 0.1 mmol L(-1)) and iMg (from 0.53 +/- 0.01 to 0.35 +/- 0.1 mmol L(-1)) was statistically significant (P < 0.001). There were no significant differences observed between the CS3000 and MCS 3p cell separators regarding the fall in Ca and Mg. None of the donors experienced any adverse reactions during the procedures. In the study, an acute ionized hypocalcaemia and hypomagnesaemia have been observed after the plateletpheresis; therefore, measurement of both the ions may be monitored. However, there is no justification for prophylactic supplementation of either of these elements.
众所周知，在血小板单采过程中，柠檬酸盐通过螯合作用诱导离子化低钙血症。然而，血清镁（Mg）离子的动力学尚未得到充分记录。因此，我们评估了血小板单采过程中健康供者的生化变化。10名健康供者在美国伊利诺伊州圆湖市百特公司的连续式血细胞分离机（CS3000）上进行血小板单采，另外10名在美敦力公司的间歇流动式血细胞分离机（MCS 3p，美国马萨诸塞州布伦特里）上进行血小板单采。在操作前、操作期间和操作后测量血清总钙和离子化钙（分别为tCa和iCa）以及镁（分别为tMg和iMg）水平。尽管tCa（从2.62±0.12降至2.36±0.12 mmol/L）和tMg（从0.89±0.01降至0.79±0.01 mmol/L）的下降幅度较小且无统计学意义；但iCa（从1.33±0.1降至0.84±0.1 mmol/L）和iMg（从0.53±0.01降至0.35±0.1 mmol/L）的下降具有统计学意义（P<0.001）。关于钙和镁的下降，在CS3000和MCS 3p血细胞分离机之间未观察到显著差异。在操作过程中，没有供者出现任何不良反应。在该研究中，血小板单采后观察到急性离子化低钙血症和低镁血症；因此，可能需要监测这两种离子。然而，预防性补充这两种元素中的任何一种都没有依据。
