Katre Uma V, Gaikwad S M, Bhagyawant S S, Deshpande U D, Khan M I, Suresh C G
Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt 1):141-3. doi: 10.1107/S1744309104032166. Epub 2004 Dec 24.
The lectin isolated from mature seeds of Cicer arietinum (CAL) agglutinates pronase-treated rabbit and human erythrocytes and its haemagglutination activity is inhibited by fetuin and desialated fetuin but not by simple monosaccharides or oligosaccharides. The purified lectin is a dimer of molecular weight 43,000 Da composed of two identical subunits (MW 21,500), as confirmed by SDS-PAGE. The lectin has been crystallized using the hanging-drop vapour-diffusion method at 295 K over a well solution containing 0.2 M sodium acetate, 0.1 M sodium phosphate buffer pH 6.5 and 14%(w/v) polyethylene glycol 8000. The triangular prism-shaped crystals belong to space group R3 and have unit-cell parameters a = b = 81.2, c = 69.4 A. The diffraction data are 93.8% complete to 2.3 A Bragg spacing with an Rmerge of 0.103.
从鹰嘴豆成熟种子中分离出的凝集素（CAL）能凝集经链霉蛋白酶处理的兔和人红细胞，其血凝活性受到胎球蛋白和去唾液酸胎球蛋白的抑制，但不受简单单糖或寡糖的抑制。经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）证实，纯化后的凝集素是一种分子量为43,000道尔顿的二聚体，由两个相同的亚基（分子量21,500）组成。该凝集素采用悬滴气相扩散法，于295 K在含有0.2 M醋酸钠、0.1 M磷酸钠缓冲液（pH 6.5）和14%（w/v）聚乙二醇8000的储液上进行结晶。三角棱柱形晶体属于R3空间群，晶胞参数a = b = 81.2，c = 69.4 Å。衍射数据在2.3 Å布拉格间距处的完整性为93.8%，合并R值为0.103。
