玫瑰旋花种子中一种甘露糖特异性凝集素的纯化、部分特性鉴定及初步X射线衍射分析
Purification, partial characterization and preliminary X-ray diffraction analysis of a mannose-specific lectin from Cymbosema roseum seeds.
作者信息
Cavada Benildo S, Marinho Emmanuel S, Souza Emmanuel P, Benevides Raquel G, Delatorre Plínio, Souza Luis A G, Nascimento Kyria S, Sampaio Alexandre H, Moreno Frederico B M B, Rustiguel Joane K R, Canduri Fernanda, de Azevedo Walter F, Debray Henri
机构信息
Department of Biochemistry, Federal University of Ceará, Brazil.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Mar 1;62(Pt 3):235-7. doi: 10.1107/S174430910600371X. Epub 2006 Feb 10.
A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour-diffusion method using a precipitant solution consisting of 0.1 M Tris-HCl pH 7.8, 8%(w/v) PEG 3350 and 0.2 M proline at a constant temperature of 293 K. A data set was collected to 1.77 A resolution at a synchrotron-radiation source. CRL crystals are orthorhombic, belonging to space group P2(1)2(1)2(1). Crystallographic refinement and full amino-acid sequence determination are in progress.
从玫瑰锦葵种子中纯化、表征并结晶出一种凝集素（CRL）。最佳晶体在一个月内生长而成，通过气相扩散法获得，沉淀剂溶液由0.1 M Tris-HCl（pH 7.8）、8%（w/v）PEG 3350和0.2 M脯氨酸组成，温度恒定为293 K。在同步辐射源处收集到分辨率为1.77 Å的数据集。CRL晶体为正交晶系，属于空间群P2(1)2(1)2(1)。晶体学精修和完整氨基酸序列测定正在进行中。
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