Won Young-Suk, Jeong Eui-Suk, Park Hyun-Ji, Lee Chul-Ho, Nam Ki-Hoan, Kim Hyoung-Chin, Hyun Byung-Hwa, Lee Sang-Koo, Choi Yang-Kyu
ICLAS Monitoring Subcenter Korea, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
Exp Anim. 2006 Jan;55(1):11-6. doi: 10.1538/expanim.55.11.
To survey the microbiological contamination of laboratory mice and rats in Korea during a 5-year period, we monitored animals housed in mouse and rat facilities with either barrier or conventional systems. At barrier and conventional mouse facilities, the most important pathogen identified was mouse hepatitis virus (MHV), while Mycoplasma pulmonis was the most important pathogen at conventional rat facilities. Interestingly, hantavirus was recovered from both barrier and conventional mouse facilities. The most common protozoon identified was Tritrichomonas muris in mouse facilities and Entamoeba muris in rat facilities. In addition, we found that the microbiological contamination of mice and rats in conventional facilities was severe. These results suggest that conventional facilities should be renovated and monitored regularly to decrease microbiological contamination. We also propose that hantavirus should be monitored in Korea as an important mouse pathogen.
为了调查韩国5年间实验小鼠和大鼠的微生物污染情况，我们监测了饲养在屏障系统或传统系统的小鼠和大鼠设施中的动物。在屏障系统和传统系统的小鼠设施中，鉴定出的最重要病原体是小鼠肝炎病毒（MHV），而在传统大鼠设施中，肺支原体是最重要的病原体。有趣的是，在屏障系统和传统系统的小鼠设施中均检测到汉坦病毒。在小鼠设施中鉴定出的最常见原生动物是鼠三毛滴虫，在大鼠设施中是鼠内阿米巴。此外，我们发现传统设施中小鼠和大鼠的微生物污染严重。这些结果表明，应定期对传统设施进行翻新和监测，以减少微生物污染。我们还建议在韩国将汉坦病毒作为一种重要的小鼠病原体进行监测。
