阿根廷常规设施中实验小鼠和大鼠的微生物污染情况。

Microbiological contaminations of laboratory mice and rats in conventional facilities in Argentina.

作者信息

Carriquiriborde Martín, Milocco Silvana, Laborde Juan Martín, Gentil Florencia, Maschi Fabricio, Principi Guido, Rogers Estela, Cagliada María Del Pilar, Ayala Miguel Angel, Carbone Cecilia

机构信息

Experimental Animal Laboratory, Faculty of Veterinary Sciences, National University of La Plata, Buenos Aires, Argentina.

出版信息

Rev Argent Microbiol. 2020 Apr-Jun;52(2):96-100. doi: 10.1016/j.ram.2019.05.003. Epub 2019 Sep 4.

DOI:10.1016/j.ram.2019.05.003

PMID:31493941

Abstract

Routine microbiological monitoring of rodent colonies in animal facilities is essential to evaluate the health status of the animals used in research studies. In the present study, animals were examined for the presence of selected microbial infections. In order to determine the contamination rates of mice and rats in Argentina, animals from 102 conventional facilities were monitored from 2012 to 2016. The most frequent bacteria isolated were Pseudomonas aeruginosa and Proteus spp. The common parasites identified were Syphacia spp. and Tritrichomonas spp. Serological assays demonstrated the highest prevalence for Mouse hepatitis virus in mice and Sialodacryoadenitis virus in rats. The results indicate that there is a high incidence of infections, so it is suggested that an efficient management system and effective sanitary barriers should be implemented in conventional facilities in Argentina in order to improve sanitary standards.

摘要

对动物设施中的啮齿动物群落进行常规微生物监测对于评估用于研究的动物的健康状况至关重要。在本研究中，对动物进行了特定微生物感染的检测。为了确定阿根廷小鼠和大鼠的污染率，2012年至2016年对来自102个传统设施的动物进行了监测。分离出的最常见细菌是铜绿假单胞菌和变形杆菌属。鉴定出的常见寄生虫是管状线虫属和三毛滴虫属。血清学检测表明，小鼠肝炎病毒在小鼠中的流行率最高，涎泪腺炎病毒在大鼠中的流行率最高。结果表明感染发生率很高，因此建议在阿根廷的传统设施中实施有效的管理系统和有效的卫生屏障，以提高卫生标准。