Shapir Errez, Cohen Hezy, Borovok Natalia, Kotlyar Alexander B, Porath Danny
Physical Chemistry Department, The Hebrew University, Jerusalem 91904, Israel.
J Phys Chem B. 2006 Mar 9;110(9):4430-3. doi: 10.1021/jp0569638.
High-resolution scanning tunneling microscopy (STM) imaging of single double-stranded poly(G)-poly(C) DNA molecules, made by a novel synthesis method, shows the molecules morphology. The STM images reveal a periodic structure of approximately 4 nm, seen as repeating "bulbs" along the molecules. These "bulbs" are associated with the molecule helix (the major grooves). "Nicks", two per 100 nm on the average, are observed along the DNA as well. The STM measurements were supported by a morphological statistics of the DNA molecule groove length and apparent height relative to the surface.
通过一种新型合成方法制备的单双链聚（G）-聚（C）DNA分子的高分辨率扫描隧道显微镜（STM）成像显示了分子形态。STM图像揭示了约4纳米的周期性结构，表现为沿分子重复出现的“球状物”。这些“球状物”与分子螺旋（大沟）相关。平均每100纳米还观察到沿DNA存在两个“切口”。STM测量得到了DNA分子沟长度和相对于表面的表观高度的形态统计数据的支持。
