全反式维甲酸对培养的人胰腺癌细胞的抗肿瘤作用。
Antitumor effects of all-trans-retinoic acid on cultured human pancreatic cancer cells.
作者信息
Guo Junming, Xiao Bingxiu, Lou Yanru, Yan Chunhong, Zhan Li, Wang Donghui, Zhao Weihong
机构信息
Ningbo University School of Medicine, Ningbo, China.
出版信息
J Gastroenterol Hepatol. 2006 Feb;21(2):443-8. doi: 10.1111/j.1440-1746.2006.04180.x.
BACKGROUND AND AIM
Although it is uncommon, pancreatic cancer is known to have a poor prognosis. The aim of the present study was to determine the inhibitory effects of all-trans-retinoic acid (ATRA) on cell growth, cell cycle and alkaline phosphatase (ALP) activity in the human pancreatic cancer cell line PANC-1 in vitro.
METHODS
Human pancreatic cancer PANC-1 cells were treated by various concentrations of ATRA, and then the cell growth was determined by MTT viability assay. Cell cycle distribution and ALP activity were analyzed by flow cytometry and chemical analyzer, respectively.
RESULTS
ATRA inhibited the growth of PANC-1 cells grown in culture; a dose-dependent inhibitory influence was found. ATRA arrested PANC-1 cells at G2/M phase. The ALP activity of PANC-1 cells was significantly increased by 1-50 micromol/L ATRA.
CONCLUSIONS
The antitumor effects of ATRA on human pancreatic cancer cells are associated with G2/M phase arrest and increased ALP activity.
背景与目的
尽管胰腺癌并不常见，但已知其预后较差。本研究的目的是确定全反式维甲酸（ATRA）对人胰腺癌细胞系PANC-1体外细胞生长、细胞周期及碱性磷酸酶（ALP）活性的抑制作用。
方法
用不同浓度的ATRA处理人胰腺癌细胞PANC-1，然后通过MTT活力测定法确定细胞生长情况。分别通过流式细胞术和化学分析仪分析细胞周期分布及ALP活性。
结果
ATRA抑制培养的PANC-1细胞生长；发现有剂量依赖性抑制作用。ATRA使PANC-1细胞停滞于G2/M期。1 - 50微摩尔/升的ATRA可显著提高PANC-1细胞的ALP活性。
结论
ATRA对人胰腺癌细胞的抗肿瘤作用与G2/M期停滞及ALP活性增加有关。
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