色素性荨麻疹（皮肤肥大细胞增多症）中人类皮肤肥大细胞的免疫组织化学特征

Immunohistochemical characterization of human cutaneous mast cells in urticaria pigmentosa (cutaneous mastocytosis).

作者信息

Akiyama M, Watanabe Y, Nishikawa T

机构信息

Department of Dermatology, Keio University School of Medicine, Tokyo, Japan.

出版信息

Acta Pathol Jpn. 1991 May;41(5):344-9. doi: 10.1111/j.1440-1827.1991.tb01657.x.

DOI:10.1111/j.1440-1827.1991.tb01657.x

PMID:1651041

Abstract

To clarify the origin and function of human cutaneous mast cells (CMCs), immunohistochemical characterization was done in 19 cases of urticaria pigmentosa (cutaneous mastocytosis) using 9 antibodies (anti-leukocyte common antigen, MX-PanB, anti-lysozyme, anti- alpha 1-antitrypsin, anti- alpha 1-antichymotrypsin, anti-vimentin, anti-neuron-specific enolase, anti-factor VIII-related antigen, and anti-ACTH). CMCs showed positive reactions with anti-alpha 1-antichymotrypsin and anti-vimentin in almost all of the specimens. In more than half of the specimens, CMCs were stained positively with anti-alpha 1-antitrypsin, MX-PanB, and anti-factor VIII-related antigen. Anti-leukocyte common antigen and anti-ACTH also showed positive reactions in some specimens. These results confirm the existence of vimentin filaments in CMCs and suggest a functional role of CMCs in hemostasis via factor VIII. Furthermore, immunohistochemical similarity between CMCs and granulocyte/macrophage-group cells is also suggested.

摘要

为阐明人类皮肤肥大细胞（CMC）的起源和功能，我们使用9种抗体（抗白细胞共同抗原、MX - PanB、抗溶菌酶、抗α1 - 抗胰蛋白酶、抗α1 - 抗糜蛋白酶、抗波形蛋白、抗神经元特异性烯醇化酶、抗因子VIII相关抗原和抗促肾上腺皮质激素）对19例色素性荨麻疹（皮肤肥大细胞增多症）进行了免疫组织化学特征分析。几乎在所有标本中，CMC对抗α1 - 抗糜蛋白酶和抗波形蛋白呈阳性反应。在超过半数的标本中，CMC对抗α1 - 抗胰蛋白酶、MX - PanB和抗因子VIII相关抗原呈阳性染色。抗白细胞共同抗原和抗促肾上腺皮质激素在一些标本中也呈阳性反应。这些结果证实了CMC中波形蛋白丝的存在，并提示CMC通过因子VIII在止血过程中发挥功能作用。此外，还提示了CMC与粒细胞/巨噬细胞组细胞之间的免疫组织化学相似性。