来自嗜热栖热菌K1的一种蛋白质二硫键氧化还原酶的结晶及初步X射线衍射研究。
Crystallization and preliminary X-ray diffraction studies of a protein disulfide oxidoreductase from Aeropyrum pernix K1.
作者信息
D'Ambrosio Katia, De Simone Giuseppina, Pedone Emilia, Rossi Mosè, Bartolucci Simonetta, Pedone Carlo
机构信息
Dipartimento di Chimica Biologica-Sezione Biostrutture, Istituto di Biostrutture e Bioimmagini-CNR, University of Naples Federico II, Via Mezzocannone 16, 80134 Naples, Italy.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Mar 1;61(Pt 3):335-6. doi: 10.1107/S1744309105004057.
Abstract
A protein disulfide oxidoreductase from the archaeon Aeropyrum pernix K1 has been overexpressed in Escherichia coli and crystallized at 298 K using the hanging-drop vapour-diffusion method. Crystals belong to the space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 90.59, b = 102.43, c = 128.96 A. A complete data set has been collected at the Elettra synchrotron source in Trieste to 1.93 A resolution using a single frozen crystal.
摘要
来自嗜热栖热放线菌K1的一种蛋白质二硫键氧化还原酶已在大肠杆菌中过表达，并采用悬滴气相扩散法于298 K下结晶。晶体属于空间群I222或I2(1)2(1)2(1)，晶胞参数a = 90.59，b = 102.43，c = 128.96 Å。使用单个冷冻晶体在的里雅斯特的Elettra同步加速器源收集了完整的数据集，分辨率达到1.93 Å。
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