产甲烷古菌詹氏甲烷球菌的全基因组序列

Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.

作者信息

Bult C J, White O, Olsen G J, Zhou L, Fleischmann R D, Sutton G G, Blake J A, FitzGerald L M, Clayton R A, Gocayne J D, Kerlavage A R, Dougherty B A, Tomb J F, Adams M D, Reich C I, Overbeek R, Kirkness E F, Weinstock K G, Merrick J M, Glodek A, Scott J L, Geoghagen N S, Venter J C

机构信息

Microbiology Department, University of Illinois, Champaign-Urbana, IL 61801, USA.

出版信息

Science. 1996 Aug 23;273(5278):1058-73. doi: 10.1126/science.273.5278.1058.

DOI:10.1126/science.273.5278.1058

PMID:8688087

Abstract

The complete 1.66-megabase pair genome sequence of an autotrophic archaeon, Methanococcus jannaschii, and its 58- and 16-kilobase pair extrachromosomal elements have been determined by whole-genome random sequencing. A total of 1738 predicted protein-coding genes were identified; however, only a minority of these (38 percent) could be assigned a putative cellular role with high confidence. Although the majority of genes related to energy production, cell division, and metabolism in M. jannaschii are most similar to those found in Bacteria, most of the genes involved in transcription, translation, and replication in M. jannaschii are more similar to those found in Eukaryotes.

摘要

通过全基因组随机测序，已经确定了自养古菌詹氏甲烷球菌完整的166万个碱基对基因组序列及其5.8和1.6万个碱基对的染色体外元件。共鉴定出1738个预测的蛋白质编码基因；然而，其中只有少数（38%）能够被高度可靠地赋予假定的细胞功能。虽然詹氏甲烷球菌中与能量产生、细胞分裂和代谢相关的大多数基因与细菌中的基因最为相似，但詹氏甲烷球菌中参与转录、翻译和复制的大多数基因与真核生物中的基因更为相似。