Ferreira R R, Cavada B S, Moreira R A, Oliveira J T, Gomes J C
Department of Pharmacology, Institute of Biosciences, Botucatu SP, Brazil.
Inflamm Res. 1996 Sep;45(9):442-7. doi: 10.1007/BF02252314.
We have characterized the histamine releasing effects of lectins extracted from Brazilian beans, in comparison to concanavalin A, in hamster cheek pouch cell suspensions containing mast cells. The lectins from Dioclea virgata, Canavalia brasiliensis, and Dioclea rostrata induce histamine release in a similar manner to concanavalin A, but appear to differ in potency and efficacy. The effects depended on the temperature, pH, and metabolic energy, demonstrating the non-cytotoxic nature of the histamine release. It is suggested that the lectins studied act by the same mechanism as concanavalin A (interacting with sugars in the antibodies bound to the mast cells), since high concentrations of glucose inhibit the histamine release. The lectins at high concentrations quench the histamine release. This suppression is reversed by increasing calcium concentration, suggesting that the lectins bind to the calcium that is essential for the secretion, thereby confirming and extending our previous data using the lectin from Dioclea virgata in rat peritoneal mast cells.
我们已对从巴西豆中提取的凝集素的组胺释放效应进行了表征，与伴刀豆球蛋白A相比，在含有肥大细胞的仓鼠颊囊细胞悬液中进行了研究。来自野大豆、巴西刀豆和具喙野大豆的凝集素以与伴刀豆球蛋白A相似的方式诱导组胺释放，但在效力和效果上似乎有所不同。这些效应取决于温度、pH值和代谢能量，表明组胺释放具有非细胞毒性性质。有人提出，所研究的凝集素与伴刀豆球蛋白A的作用机制相同（与结合在肥大细胞上的抗体中的糖相互作用），因为高浓度的葡萄糖会抑制组胺释放。高浓度的凝集素会抑制组胺释放。通过增加钙浓度可逆转这种抑制作用，这表明凝集素与分泌所必需的钙结合，从而证实并扩展了我们之前使用野大豆凝集素在大鼠腹膜肥大细胞中的数据。
