Machiah Deepa K, Girish K S, Gowda T Veerabasappa
Department of Pathology, Emory School of Medicine, Atlanta, Georgia, USA.
Comp Biochem Physiol C Toxicol Pharmacol. 2006 Jun;143(2):158-61. doi: 10.1016/j.cbpc.2006.01.006. Epub 2006 Mar 2.
Venom hyaluronidases help in rapid spreading of the toxins by destroying the integrity of the extra-cellular matrix of the tissues in the victims. A hyaluronidase inhibitor (WSG) is purified from a folk medicinal plant, Withania somnifera. The glycoprotein inhibited the hyaluronidase activity of cobra (Naja naja) and viper (Daboia russelii) venoms, which was demonstrated by zymogram assay and staining of the skin tissues for differential activity. WSG completely inhibited the activity of the enzyme at a concentration of 1:1 w/w of venom to WSG. Thus we are able to demonstrate that the glycoprotein inhibits hyaluronidase activity of the venoms. External application of the plant extract as an antidote in rural parts of India to snakebite victims appears to have a scientific basis.
毒液透明质酸酶通过破坏受害者组织细胞外基质的完整性，帮助毒素快速扩散。从民间药用植物印度人参（Withania somnifera）中纯化出一种透明质酸酶抑制剂（WSG）。该糖蛋白抑制了眼镜蛇（Naja naja）和蝰蛇（Daboia russelii）毒液的透明质酸酶活性，这通过酶谱分析和对皮肤组织进行不同活性染色得以证明。WSG在毒液与WSG的重量比为1:1时完全抑制了该酶的活性。因此，我们能够证明这种糖蛋白抑制毒液的透明质酸酶活性。在印度农村地区，将该植物提取物作为蛇咬伤受害者的解毒剂外用似乎有科学依据。
