Bolduc J-E, Lewis L J, Aubin C-E, Geitmann A
Département de Physique, Université de Montréal, C.P. 6128 Succursale Centre-Ville, Montréal, QC, Canada, H3C 3J7.
Biomech Model Mechanobiol. 2006 Nov;5(4):227-36. doi: 10.1007/s10237-005-0010-1. Epub 2006 Mar 3.
Micro-indentation is a new experimental approach to assess physical cellular properties. Here we attempt to quantify the contribution of geometrical parameters to a cylindrical plant cell's resistance to lateral deformation. This information is important to correctly interpret data obtained from experiments using the device, such as the local cellular stiffness in pollen tubes. We built a simple finite-element model of the micro-indentation interacting partners - micro-indenter, cell (pollen tube), and underlying substratum, that allowed us to manipulate geometric variables, such as geometry of the cell, cell radius, thickness of the cell wall and radius of the indenting stylus. Performing indentation experiments on this theoretical model demonstrates that all four parameters influence stiffness measurement and can therefore not be neglected in the interpretation of micro-indentation data.
微压痕是一种评估细胞物理特性的新实验方法。在此，我们试图量化几何参数对圆柱形植物细胞抗侧向变形能力的贡献。这些信息对于正确解释使用该装置进行实验所获得的数据非常重要，例如花粉管中的局部细胞刚度。我们构建了一个微压痕相互作用对象（微压头、细胞（花粉管）和下层基质）的简单有限元模型，该模型使我们能够操纵几何变量，如细胞的几何形状、细胞半径、细胞壁厚度和压痕探针半径。对该理论模型进行压痕实验表明，所有这四个参数都会影响刚度测量，因此在解释微压痕数据时不能忽视。
