Ffrench-Constant R H, Mortlock D P, Shaffer C D, MacIntyre R J, Roush R T
Department of Entomology, Russell Laboratories, University of Wisconsin, Madison 53706.
Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7209-13. doi: 10.1073/pnas.88.16.7209.
Cyclodiene resistance represents 60% of the reported cases of insecticide resistance and is also present in vertebrates. Resistance is due to insensitivity of the cyclodiene/picrotoxinin binding site on the gamma-aminobutyric acid subtype A (GABAA) receptor-chloride ionophore complex. Following isolation of cyclodiene-resistant Drosophila mutants, we report the cloning of the locus conferring resistance via a "chromosomal walk" and rescue of the susceptible phenotype by P-element-mediated germ-line transformation. Amino acid sequence analysis of a cDNA from the locus reveals homology with vertebrate GABAA subunits. To our knowledge, this represents the first cloning of an invertebrate GABA receptor and also allows us to manipulate the resistance status of an insect via germ-line transformation. This gene may be useful as a selectable marker in other insect systems.
环二烯抗性占已报道的杀虫剂抗性案例的60%，在脊椎动物中也存在。抗性是由于γ-氨基丁酸A型（GABAA）受体-氯离子通道复合物上环二烯/印防己毒素结合位点不敏感所致。在分离出抗环二烯的果蝇突变体后，我们报告了通过“染色体步移”克隆赋予抗性的基因座，并通过P因子介导的种系转化挽救了敏感表型。对该基因座的cDNA进行氨基酸序列分析，发现其与脊椎动物GABAA亚基具有同源性。据我们所知，这是首次克隆无脊椎动物GABA受体，也使我们能够通过种系转化来操控昆虫的抗性状态。该基因可能作为其他昆虫系统中的选择标记有用。
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