Bialkowska-Hobrzanska Hanna, Driman David K, Fletcher Robert, Harry Vimla, Razvi Hassan
Department of Microbiology & Immunology, The University of Western Ontario and Lawson Research Institute, London, Ontario, Canada.
Can J Urol. 2006 Feb;13(1):2967-74.
The wide spectrum of biological behavior displayed by prostate cancer (PCa) warrants investigation of potential PCa-specific biomarkers that could identify more aggressive tumor types and therefore provide prognostic value. Upregulation of expression of human telomerase reverse transcriptase (hTERT), Survivin, DD3 and PCGEM1 mRNAs in PCa lesions has recently been described. The purpose of this study was to evaluate the clinical value of detection of over-expression of these biomarkers in the diagnosis and prognosis of PCa.
Archival formalin-fixed, paraffin-embedded (FFPE) prostatectomy tissue from 26 patients with PCa (Gleason score 3-9, mean 7) and 14 patients with benign prostatic hyperplasia (BPH) were analyzed by reverse transcription polymerase chain reaction (RT-PCR) for semiquantitative transcript levels of hTERT, Survivin, DD3 and PCGEM1. In addition, 25 matched normal (MN) tissue samples were examined. The expression of biomarker mRNA relative to b2-microglobulin mRNA was determined using AlphaImager 2200 data analysis software.
The biomarkers had sensitivities ranging from 91% to 100%. Clinical specificities evaluated with the BPH tissue were the following: hTERT mRNA (93%), DD3 mRNA (57%), Survivin (29%) and PCGEM1 (14%). Biomarker expressions were up to 13.5-fold higher in PCa tissue as compared to MN tissue. None of the tumor biomarkers showed a positive correlation with pathological stage and Gleason score.
The results of this study indicate potential utility of the hTERT mRNA and DD3 mRNA as diagnostic but not prognostic biomarkers for PCa.
前列腺癌（PCa）所表现出的广泛生物学行为，使得对潜在的PCa特异性生物标志物进行研究成为必要，这些标志物能够识别更具侵袭性的肿瘤类型，从而提供预后价值。最近已有报道称，PCa病变中人类端粒酶逆转录酶（hTERT）、生存素、DD3和PCGEM1 mRNA的表达上调。本研究的目的是评估检测这些生物标志物的过表达在PCa诊断和预后中的临床价值。
采用逆转录聚合酶链反应（RT-PCR）对26例PCa患者（Gleason评分3 - 9，平均7分）和14例良性前列腺增生（BPH）患者的存档福尔马林固定、石蜡包埋（FFPE）前列腺切除组织进行分析，以半定量检测hTERT、生存素、DD3和PCGEM1的转录水平。此外，还检测了25份匹配的正常（MN）组织样本。使用AlphaImager 2200数据分析软件测定生物标志物mRNA相对于β2-微球蛋白mRNA的表达。
这些生物标志物的敏感性范围为91%至100%。用BPH组织评估的临床特异性如下：hTERT mRNA（93%）、DD3 mRNA（57%）、生存素（29%）和PCGEM1（14%）。与MN组织相比，PCa组织中生物标志物的表达高出13.5倍。没有一种肿瘤生物标志物与病理分期和Gleason评分呈正相关。
本研究结果表明，hTERT mRNA和DD3 mRNA作为PCa的诊断生物标志物具有潜在效用，但不具有预后价值。
