DD3（PCA3）基因在癌症和前列腺增生中的表达。
DD3(PCA3) gene expression in cancer and prostatic hyperplasia.
作者信息
Floriano-Sánchez E, Cárdenas-Rodríguez N, Castro-Marín M, Alvarez-Grave P, Lara-Padilla E
机构信息
Departamento de Bioquímica y Biología Molecular, Escuela Médico Militar, México, DF.
出版信息
Clin Invest Med. 2009 Dec 1;32(6):E258.
PURPOSE
DD3(PCA3) is a novel gene with characteristics that indicate its potentially valuable role in early identification and diagnosis of malignancy and highly upregulated in transformed cells in PCa. The aim of this work was to validate and analyze, by real-time Reverse Transcription-Polymerase Chain Reaction (RT-PCR), the expression of the DD3(PCA3)gene in a Mexican population, both in intratumoral tissue with PCa and benign prostatic hyperplasia (BPH).
METHODS
Human samples from patients with PCa (40 cases) and benign prostatic hyperplasia (40 cases) were analyzed for the mRNA expression of DD3(PCA3)by RT-PCR RESULTS: The GAPDH gene showed better stability with a Pearson correlation of 0.953 (P < 0.007) for the determination of housekeeping gene. DD3(PCA3)gene expression was 29.74 times higher in PCa tissue (P < 0.0001) than in BPH. The gene expression for the PCa and BPH was 1731+/-280 and 58.23+/-9.9 fold, respectively.
CONCLUSIONS
Determination of DD3(PCA3)gene expression by RT-PCR could be a potentially tool for the early detection of PCa in clinical specimens.
目的
DD3（PCA3）是一种新型基因，其特性表明它在恶性肿瘤的早期识别和诊断中可能具有重要价值，并且在前列腺癌（PCa）的转化细胞中高度上调。本研究旨在通过实时逆转录-聚合酶链反应（RT-PCR）验证和分析DD3（PCA3）基因在墨西哥人群中的表达情况，包括前列腺癌肿瘤组织和良性前列腺增生（BPH）组织。
方法
采用RT-PCR技术分析40例前列腺癌患者和40例良性前列腺增生患者的样本中DD3（PCA3）的mRNA表达。
结果
对于看家基因的测定，GAPDH基因显示出更好的稳定性，Pearson相关性为0.953（P < 0.007）。DD3（PCA3）基因在前列腺癌组织中的表达比良性前列腺增生组织高29.74倍（P < 0.0001）。前列腺癌和良性前列腺增生的基因表达分别为1731±280和58.23±9.9倍。
结论
通过RT-PCR测定DD3（PCA3）基因表达可能是临床标本中早期检测前列腺癌的潜在工具。
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