Burneiko Regina C M, Diniz Yeda S, Galhardi Cristiano M, Rodrigues Hosana G, Ebaid Geovana M X, Faine Luciane A, Padovani Carlos R, Cicogna Antonio C, Novelli Ethel L B
Department of Chemistry and Biochemistry, Institute of Biological Sciences, São Paulo State University, UNESP, 18618-000 Botucatu, São Paulo, Brazil.
Food Chem Toxicol. 2006 Jul;44(7):1167-72. doi: 10.1016/j.fct.2006.01.004. Epub 2006 Mar 3.
The present study examined the interaction of hypercaloric diet (HD) and physical exercise on lipid profile and oxidative stress in serum and liver of rats. Male Wistar rats (60-days-old) were fed with a control (C) and hypercaloric diet (H). Each of the two dietary groups (C and H) was divided into three subgroups (n=8), sedentary (CS and HS), exercised 2days a week (CE2 and HE2) and exercised 5days a week (CE5 and HE5). The swimming was selected as a model for exercise performance. After 8-weeks exercised rats showed decreased lactate dehydrogenase serum activities, demonstrating the effectiveness of the swimming as an aerobic-training protocol. Exercise 5-days a week reduced the body weight gain. Triacylglycerol (TG) and very low-density lipoprotein (VLDL-C) were increased in HD-fed rats. HE5 and CE5 rats had decreased TG, VLDL-C and cholesterol. HE2 rats had enhanced high-density lipoprotein (HDL-C) in serum. No alterations were observed in lipid hydroperoxide (LH), while total antioxidant substances (TAS) were increased in serum of exercised rats. HD-fed rats had hepatic TG accumulation. Superoxide dismutase activities were increased and catalase was decreased in liver of exercised rats. The interaction of HD and physical exercise reduced TAS and enhanced LH levels in hepatic tissue. In conclusion, this study confirmed the beneficial effect of physical exercise as a dyslipidemic-lowering component. Interaction of HD and physical exercise had discrepant effects on serum and liver oxidative stress. The interaction of HD and physical exercise reduced the oxidative stress in serum. HD and physical exercise interaction had pro-oxidant effect on hepatic tissue, suggesting that more studies should be done before using physical exercise as an adjunct therapy to reduce the adverse effects of HD.
本研究考察了高热量饮食（HD）与体育锻炼对大鼠血清和肝脏脂质谱及氧化应激的相互作用。将60日龄雄性Wistar大鼠分为对照组（C）和高热量饮食组（H）。两个饮食组（C和H）各自又分为三个亚组（n = 8），即久坐组（CS和HS）、每周锻炼2天组（CE2和HE2）以及每周锻炼5天组（CE5和HE5）。选择游泳作为运动方式。8周的运动后，大鼠血清乳酸脱氢酶活性降低，表明游泳作为有氧训练方案是有效的。每周锻炼5天可减少体重增加。喂食HD的大鼠三酰甘油（TG）和极低密度脂蛋白（VLDL-C）升高。HE5组和CE5组大鼠的TG、VLDL-C和胆固醇降低。HE2组大鼠血清高密度脂蛋白（HDL-C）升高。脂质氢过氧化物（LH）未观察到变化，而运动大鼠血清中总抗氧化物质（TAS）增加。喂食HD的大鼠肝脏有TG蓄积。运动大鼠肝脏中超氧化物歧化酶活性增加，过氧化氢酶活性降低。HD与体育锻炼的相互作用使肝组织中TAS降低，LH水平升高。总之，本研究证实了体育锻炼作为降血脂成分的有益作用。HD与体育锻炼的相互作用对血清和肝脏氧化应激有不同影响。HD与体育锻炼的相互作用降低了血清中的氧化应激。HD与体育锻炼的相互作用对肝组织有促氧化作用，这表明在将体育锻炼用作辅助治疗以减少HD的不良影响之前，应进行更多研究。
