非洲爪蟾卵母细胞中表达的电压依赖性钾通道的递质调节。
Transmitter regulation of voltage-dependent K+ channels expressed in Xenopus oocytes.
作者信息
Kavanaugh M P, Christie M J, Osborne P B, Busch A E, Shen K Z, Wu Y N, Seeburg P H, Adelman J P, North R A
机构信息
Vollum Institute, Oregon Health Sciences University, Portland 97201.
出版信息
Biochem J. 1991 Aug 1;277 ( Pt 3)(Pt 3):899-902. doi: 10.1042/bj2770899.
Abstract
Voltage-dependent K+ channels (RBK1, RBK2 and RGK5) were co-expressed in Xenopus oocytes with 5-hydroxytryptamine (5-HT2) receptors. K+ currents measured 2-4 days later were inhibited by 5-HT (100 nM-10 microM, 20-30 s application) by up to 90%. The effect of 5-HT was mimicked by intracellular injection of Ins(1,4,5)P3. Increasing the Ca2+ concentration at the inner surface of excised membrane patches did not decrease the K+ current.
摘要
电压依赖性钾通道（RBK1、RBK2和RGK5）与5-羟色胺（5-HT2）受体在非洲爪蟾卵母细胞中共同表达。2 - 4天后测量的钾电流被5-羟色胺（100 nM - 10 microM，施加20 - 30秒）抑制高达90%。细胞内注射肌醇三磷酸（Ins(1,4,5)P3）可模拟5-羟色胺的作用。增加切除膜片内表面的钙离子浓度并不会降低钾电流。
相似文献
1
Transmitter regulation of voltage-dependent K+ channels expressed in Xenopus oocytes.非洲爪蟾卵母细胞中表达的电压依赖性钾通道的递质调节。
Biochem J. 1991 Aug 1;277 ( Pt 3)(Pt 3):899-902. doi: 10.1042/bj2770899.
2
Heteropolymeric potassium channels expressed in Xenopus oocytes from cloned subunits.由克隆亚基在非洲爪蟾卵母细胞中表达的异聚体钾通道。
Neuron. 1990 Mar;4(3):405-11. doi: 10.1016/0896-6273(90)90052-h.
3
Regulation of inositol trisphosphate-induced membrane currents in Xenopus oocytes by a Jurkat cell calcium influx factor.Jurkat细胞钙内流因子对非洲爪蟾卵母细胞中肌醇三磷酸诱导的膜电流的调节作用
Biochem J. 1996 Sep 1;318 ( Pt 2)(Pt 2):649-56. doi: 10.1042/bj3180649.
4
Regulation by second messengers of the slowly activating, voltage-dependent potassium current expressed in Xenopus oocytes.
J Physiol. 1992 May;450:491-502. doi: 10.1113/jphysiol.1992.sp019138.
5
Receptors of the serotonin 1C subtype expressed from cloned DNA mediate the closing of K+ membrane channels encoded by brain mRNA.从克隆DNA表达的5-羟色胺1C亚型受体介导由脑信使核糖核酸编码的钾离子膜通道的关闭。
Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2560-2. doi: 10.1073/pnas.88.6.2560.
6
A delayed rectifier potassium current in Xenopus oocytes.非洲爪蟾卵母细胞中的延迟整流钾电流。
Biophys J. 1990 Jun;57(6):1117-23. doi: 10.1016/S0006-3495(90)82632-1.
7
Interaction between capacitative Ca2+ influx and Ca2+-dependent Cl- currents in Xenopus oocytes.
Pflugers Arch. 1995 Oct;430(6):954-63. doi: 10.1007/BF01837409.
8
Latencies of membrane currents evoked in Xenopus oocytes by receptor activation, inositol trisphosphate and calcium.在非洲爪蟾卵母细胞中，由受体激活、三磷酸肌醇和钙所诱发的膜电流潜伏期。
J Physiol. 1989 Aug;415:189-210. doi: 10.1113/jphysiol.1989.sp017718.
9
Serotonin receptors expressed in Xenopus oocytes by mRNA from brain mediate a closing of K+ membrane channels.由来自大脑的信使核糖核酸在非洲爪蟾卵母细胞中表达的血清素受体介导钾离子膜通道的关闭。
Brain Res Mol Brain Res. 1990 Jan;7(1):31-8. doi: 10.1016/0169-328x(90)90070-t.
10
Synergism of inositol trisphosphate and tetrakisphosphate in activating Ca2+-dependent K+ channels.肌醇三磷酸和四磷酸在激活钙依赖性钾通道中的协同作用。
Nature. 1987;330(6149):653-5. doi: 10.1038/330653a0.
引用本文的文献
1
5-HT2 receptors-mediated modulation of voltage-gated K+ channels and neurophysiopathological correlates.5-HT2 受体介导的电压门控 K+通道调制与神经生理病理相关性。
Exp Brain Res. 2013 Oct;230(4):453-62. doi: 10.1007/s00221-013-3555-8. Epub 2013 May 24.
2
Five ADNFLE mutations reduce the Ca2+ dependence of the mammalian alpha4beta2 acetylcholine response.五个常染色体显性夜间额叶癫痫（ADNFLE）突变降低了哺乳动物α4β2型乙酰胆碱反应对钙离子的依赖性。
J Physiol. 2003 Jul 1;550(Pt 1):11-26. doi: 10.1113/jphysiol.2003.036681. Epub 2003 May 16.
3
Serotonin and protein kinase C modulation of a rat brain inwardly rectifying K+ channel expressed in xenopus oocytes.5-羟色胺和蛋白激酶C对非洲爪蟾卵母细胞中表达的大鼠脑内向整流钾通道的调节作用
Pflugers Arch. 1996 Jan;431(3):335-40. doi: 10.1007/BF02207270.
4
Regulation by second messengers of the slowly activating, voltage-dependent potassium current expressed in Xenopus oocytes.
J Physiol. 1992 May;450:491-502. doi: 10.1113/jphysiol.1992.sp019138.
本文引用的文献
1
Protein kinase injection reduces voltage-dependent potassium currents.蛋白激酶注射可降低电压依赖性钾电流。
Science. 1983 Jan 21;219(4582):303-6. doi: 10.1126/science.6294830.
2
Rapid and efficient site-specific mutagenesis without phenotypic selection.无需表型筛选的快速高效位点特异性诱变。
Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92. doi: 10.1073/pnas.82.2.488.
3
Cloning of a membrane protein that induces a slow voltage-gated potassium current.一种可诱导缓慢电压门控钾电流的膜蛋白的克隆。
Science. 1988 Nov 18;242(4881):1042-5. doi: 10.1126/science.3194754.
4
Effect of W7 on Ca2+ uptake and Ca2+-ATPase activities of the endoplasmic reticulum of rat liver.W7对大鼠肝脏内质网Ca2+摄取及Ca2+-ATP酶活性的影响。
Arch Biochem Biophys. 1987 Aug 1;256(2):472-9. doi: 10.1016/0003-9861(87)90604-7.
5
Long-term potentiation of synaptic transmission in the hippocampus induced by a bee venom peptide.蜂毒肽诱导海马体突触传递的长期增强作用。
Nature. 1987;328(6125):70-3. doi: 10.1038/328070a0.
6
Structure and functional expression of cloned rat serotonin 5HT-2 receptor.克隆大鼠血清素5HT - 2受体的结构与功能表达
EMBO J. 1988 Dec 20;7(13):4135-40. doi: 10.1002/j.1460-2075.1988.tb03308.x.
7
Protein kinase C activators block specific calcium and potassium current components in isolated hippocampal neurons.蛋白激酶C激活剂可阻断离体海马神经元中特定的钙电流和钾电流成分。
J Neurosci. 1988 Nov;8(11):4069-78. doi: 10.1523/JNEUROSCI.08-11-04069.1988.
8
cDNA cloning of bovine substance-K receptor through oocyte expression system.通过卵母细胞表达系统克隆牛速激肽K受体的cDNA
Nature. 1987;329(6142):836-8. doi: 10.1038/329836a0.
9
Modulation of the delayed rectifier K+ current by isoprenaline in bull-frog atrial myocytes.异丙肾上腺素对牛蛙心房肌细胞延迟整流钾电流的调制作用
J Physiol. 1989 Aug;415:233-49. doi: 10.1113/jphysiol.1989.sp017720.
10
Cyclic AMP-dependent protein kinase closes the serotonin-sensitive K+ channels of Aplysia sensory neurones in cell-free membrane patches.环磷酸腺苷依赖性蛋白激酶可关闭海兔感觉神经元无细胞膜片中对5-羟色胺敏感的钾通道。
Nature. 1985;313(6001):392-5. doi: 10.1038/313392a0.
Zotero 插件