Takumi T, Ohkubo H, Nakanishi S
Institute for Immunology, Kyoto University Faculty of Medicine, Japan.
Science. 1988 Nov 18;242(4881):1042-5. doi: 10.1126/science.3194754.
A rat kidney messenger RNA that induces a slowly activating, voltage-dependent potassium current on its expression in Xenopus oocytes was identified by combining molecular cloning with an electrophysiological assay. The cloned complementary DNA encodes a novel membrane protein that consists of 130 amino acids with a single putative transmembrane domain. This protein differs from the known ion channel proteins but is involved in the induction of selective permeation of potassium ions by membrane depolarization.
通过将分子克隆与电生理检测相结合，鉴定出一种大鼠肾脏信使核糖核酸（mRNA），该mRNA在非洲爪蟾卵母细胞中表达时可诱导一种缓慢激活的电压依赖性钾电流。克隆的互补DNA编码一种新型膜蛋白，该蛋白由130个氨基酸组成，具有一个单一的假定跨膜结构域。这种蛋白不同于已知的离子通道蛋白，但参与通过膜去极化诱导钾离子的选择性通透。
