从克隆DNA表达的5-羟色胺1C亚型受体介导由脑信使核糖核酸编码的钾离子膜通道的关闭。
Receptors of the serotonin 1C subtype expressed from cloned DNA mediate the closing of K+ membrane channels encoded by brain mRNA.
作者信息
Panicker M M, Parker I, Miledi R
机构信息
Department of Psychobiology, University of California, Irvine 92717.
出版信息
Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2560-2. doi: 10.1073/pnas.88.6.2560.
Abstract
The modulation of K+ channels by serotonin (5-HT) receptors was studied by coinjecting Xenopus oocytes with mRNA transcribed in vitro from a cloned 5-HT 1C subtype (5-HT1C) receptor gene, together with size-fractionated mRNA isolated from rat cerebral cortex that expresses K+ channels. After intracellular loading with EGTA to block Ca2(+)-dependent chloride currents, these oocytes responded to 5-HT with an inward current associated with a decrease in membrane conductance. Membrane current responses were small or absent in oocytes injected with either mRNA alone. We conclude that 5-HT1C receptors are able to cause the closing of a class of K+ channels expressed by cortex mRNA in a Ca2(+)-independent manner. The coupling between the receptors and channels appears to be mediated by the inositol phospholipid second messenger pathway, since activation of this pathway by application of serum evoked a similar closing current.
摘要
通过将体外转录自克隆的5-羟色胺1C亚型（5-HT1C）受体基因的mRNA与从表达钾通道的大鼠大脑皮层分离的大小分级mRNA共同注射到非洲爪蟾卵母细胞中，研究了5-羟色胺（5-HT）受体对钾通道的调节作用。在用乙二醇双四乙酸（EGTA）进行细胞内加载以阻断钙依赖性氯电流后，这些卵母细胞对5-羟色胺产生内向电流，伴随着膜电导降低。单独注射任一mRNA的卵母细胞中膜电流反应很小或无反应。我们得出结论，5-HT1C受体能够以不依赖钙的方式导致由皮层mRNA表达的一类钾通道关闭。受体与通道之间的偶联似乎是由肌醇磷脂第二信使途径介导的，因为应用血清激活该途径会诱发类似的关闭电流。
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