Bagavandoss P, Wilks J W
Cancer and Infectious Diseases Research, Upjohn Laboratories, Kalamazoo, Michigan 49001.
Biol Reprod. 1991 Jun;44(6):1132-9. doi: 10.1095/biolreprod44.6.1132.
We have isolated and characterized microvascular endothelial cells from the developing rabbit corpus luteum. The isolated cells express Factor VIII-related antigen and angiotensin-converting enzyme, internalize acetylated low-density lipoprotein, and form capillary-like tubules in collagen gel cultures. Of the mitogens tested, only basic fibroblast growth factor stimulated the proliferation of these cells. Transforming growth factor-beta 1 and tumor necrosis factor-alpha strongly inhibited the proliferation of these endothelial cells. Platelet-derived growth factor, epidermal growth factor, insulin-like growth factor-1, histamine, prostaglandins, sex steroids, and interleukin-6 (interferon-beta 2) had no effect on the proliferation of these microvascular endothelial cells from the corpus luteum, whereas interleukin-1 alpha and 1 beta were mildly inhibitory. Endothelial cells are an essential component of corpus luteum physiology. Therefore, the availability of these cells will allow us to investigate the potential interactions between endothelial cells and luteal cells in vitro.
我们已经从发育中的兔黄体中分离并鉴定了微血管内皮细胞。分离出的细胞表达VIII因子相关抗原和血管紧张素转换酶，摄取乙酰化低密度脂蛋白，并在胶原凝胶培养物中形成毛细血管样小管。在所测试的促有丝分裂原中，只有碱性成纤维细胞生长因子能刺激这些细胞的增殖。转化生长因子-β1和肿瘤坏死因子-α强烈抑制这些内皮细胞的增殖。血小板衍生生长因子、表皮生长因子、胰岛素样生长因子-1、组胺、前列腺素、性类固醇和白细胞介素-6（干扰素-β2）对这些来自黄体的微血管内皮细胞的增殖没有影响，而白细胞介素-1α和1β有轻微抑制作用。内皮细胞是黄体生理学的重要组成部分。因此，这些细胞的获得将使我们能够在体外研究内皮细胞与黄体细胞之间潜在的相互作用。
