Stypmann Jörg, Engelen Markus A, Epping Constanze, van Rijen Harold V M, Milberg Peter, Bruch Christian, Breithardt Günter, Tiemann Klaus, Eckardt Lars
Department of Cardiology and Angiology, Hospital of the University of Münster, Münster, Germany.
Int J Cardiovasc Imaging. 2006 Jun-Aug;22(3-4):353-62. doi: 10.1007/s10554-005-9052-9. Epub 2006 Mar 4.
Doppler-echocardiography of the mouse has evolved to a commonly used technique in the past years as recent advances in imaging quality have substantially improved spatial and temporal resolution allowing the adaptation of this technique to murine models. Although mouse echocardiography is widely used, there is only little information on reference data for wild-type animals available, particularly in older mice.
We therefore established a database with echocardiographic reference-values in a large set of young (8 weeks) and older adult (52 weeks) Swiss type CD1-mice of either sex. We performed a complete Doppler-echocardiographic examination under light Ketamine-Xylazine-anesthesia. LV-mass was calculated and compared with necropsy heart weights to validate the LV-mass calculation.
Doppler-echocardiographic measurements in mice were feasible to assess cardiac morphology and function. Sonomorphological and functional parameters hardly changed between the age of 12 and 52 weeks. Wall thickness, LV-mass and cardiac output were stable with aging. There was a good relative correlation between echocardiographically estimated LV-mass and necropsy heart weight although absolute values differed. There were no significant echocardiographic differences between male and female mice.
The reference values established in this study can be useful in recording and quantifying pathological changes in murine models of cardiovascular diseases. There is hardly any change of cardiac function between the age of 12 and 52 weeks.
在过去几年中，随着成像质量的最新进展显著提高了空间和时间分辨率，使得该技术能够应用于小鼠模型，小鼠的多普勒超声心动图已发展成为一种常用技术。尽管小鼠超声心动图被广泛使用，但关于野生型动物的参考数据却很少，尤其是在老年小鼠中。
因此，我们建立了一个包含大量年轻（8周龄）和成年老年（52周龄）瑞士型CD1小鼠（雌雄均有）超声心动图参考值的数据库。我们在轻度氯胺酮-赛拉嗪麻醉下进行了完整的多普勒超声心动图检查。计算左心室质量，并与尸检心脏重量进行比较，以验证左心室质量的计算。
小鼠的多普勒超声心动图测量对于评估心脏形态和功能是可行的。在12周龄至52周龄之间，超声形态学和功能参数几乎没有变化。随着年龄增长，室壁厚度、左心室质量和心输出量保持稳定。尽管绝对值不同，但超声心动图估计的左心室质量与尸检心脏重量之间存在良好的相对相关性。雄性和雌性小鼠之间在超声心动图上没有显著差异。
本研究建立的参考值可用于记录和量化心血管疾病小鼠模型中的病理变化。在12周龄至52周龄之间，心脏功能几乎没有任何变化。
