Acton Paul D, Thomas Daniel, Zhou Rong
Department of Radiology, University of Pennsylvania, Philadelphia, PA 19107, USA.
Int J Cardiovasc Imaging. 2006 Jun-Aug;22(3-4):429-34. doi: 10.1007/s10554-005-9046-7. Epub 2006 Mar 4.
Small animal imaging of cardiovascular disease using single photon emission tomography (SPECT) can be used to provide quantitative measurements of myocardial infarct. The purpose of this study was to demonstrate the accuracy of pinhole SPECT imaging with [99mTc]sestamibi for estimation of infarct size in a rat model of coronary artery disease. Nine rats had their left anterior descending artery ligated to induce a region of myocardial infarct. These animals were injected with 37 MBq [99mTc]sestamibi, and, 1 h later, scanned on a pinhole SPECT system for 30 min. The defect size measured with SPECT, which was dependent on a threshold applied to the short axis circumferential profiles, was compared against the gold standard triphenyltetrazolium chloride (TTC) staining. The size of the perfusion deficit measured using [99mTc]sestamibi SPECT compared very favorably with the TTC staining result, for threshold values in the range 50-70%. The optimum threshold was approximately 70%, giving an excellent correlation (R2=0.89, p<0.001). Estimation of infarct size by [99mTc] sestamibi SPECT yielded an excellent agreement with TTC staining. In conclusion, measurement of myocardial infarct with SPECT can be used to study the rat heart in vivo, and provides a quantitative measure of myocardial viability.
使用单光子发射断层扫描（SPECT）对心血管疾病进行小动物成像可用于提供心肌梗死的定量测量。本研究的目的是证明用[99mTc]司他米比进行针孔SPECT成像在估计冠状动脉疾病大鼠模型中梗死面积方面的准确性。9只大鼠结扎左前降支动脉以诱导心肌梗死区域。给这些动物注射37 MBq的[99mTc]司他米比，1小时后，在针孔SPECT系统上扫描30分钟。将SPECT测量的缺损大小（取决于应用于短轴圆周轮廓的阈值）与金标准氯化三苯基四氮唑（TTC）染色结果进行比较。对于50 - 70%范围内的阈值，使用[99mTc]司他米比SPECT测量的灌注缺损大小与TTC染色结果非常吻合。最佳阈值约为70%，相关性极佳（R2 = 0.89，p < 0.001）。用[99mTc]司他米比SPECT估计梗死面积与TTC染色结果高度一致。总之，用SPECT测量心肌梗死可用于在体研究大鼠心脏，并提供心肌活力的定量测量。
