采用高效液相色谱-紫外检测法测定大鼠血浆中的米格列奈。

Determination of mitiglinide in rat plasma by high-performance liquid chromatography with UV detection.

作者信息

Lushan Yu, Su Zeng

机构信息

Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310031, China.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Apr 13;834(1-2):204-7. doi: 10.1016/j.jchromb.2006.02.029. Epub 2006 Mar 6.

DOI:10.1016/j.jchromb.2006.02.029

PMID:16520101

Abstract

A selective and sensitive high-performance liquid chromatography method has been developed and validated for determination of mitiglinide (MGN) in rat plasma using 2-(4-biphenylyl) propionic acid (BPA) as internal standard. Liquid-liquid extraction was used for sample preparation. Chromatographic separation was achieved on a C(18) column using acetonitrile and 0.02 mol/l KH(2)PO(4) buffer (pH 4.0) (45:55, v/v) as mobile phase delivered at 1.0 ml/min. The UV detector was set at 210 nm. The assay was linear over the range 0.1-20 microg/ml for MGN. The average extraction recoveries of MGN and BPA from rat plasma were 98.6 and 97.4%, respectively. The developed method has been applied to the pharmacokinetic study of MGN in rats.

摘要

已开发并验证了一种选择性和灵敏的高效液相色谱法，以2-(4-联苯基)丙酸(BPA)为内标，用于测定大鼠血浆中的米格列奈(MGN)。采用液-液萃取法进行样品制备。在C(18)柱上进行色谱分离，以乙腈和0.02 mol/l KH(2)PO(4)缓冲液(pH 4.0)(45:55, v/v)为流动相，流速为1.0 ml/min。紫外检测器设置在210 nm。该测定法在0.1-20 μg/ml范围内对MGN呈线性。MGN和BPA从大鼠血浆中的平均萃取回收率分别为98.6%和97.4%。所开发的方法已应用于MGN在大鼠体内的药代动力学研究。