Minor P D, Roscoe D H
J Cell Sci. 1975 Mar;17(3):381-96. doi: 10.1242/jcs.17.3.381.
Colchicine-resistant variants derived from mouse and Syrian hamster lines are described. The resistant cells do not appear to be true mutants, since they appear at a high frequency, unaffected by treatment with ethyl methyl sulphonate, and are unstable in the absence of the drug. They are cross-resistant to other drugs, show a reduced rate of binding of colchicine in monolayer, and give extracts with colchicine-binding properties identical to those of the wild type. Thus the resistance is due to a permeability barrier. The naturally occurring resistance of the Syrian hamster line is specific for colchicine, and may be due to a specific permeability barrier. The Syrian hamster line is also shown to have an extra colchicine-binding pool.
本文描述了从小鼠和叙利亚仓鼠细胞系中获得的秋水仙碱抗性变体。这些抗性细胞似乎并非真正的突变体，因为它们出现的频率很高，不受甲基磺酸乙酯处理的影响，并且在无药物的情况下不稳定。它们对其他药物具有交叉抗性，在单层培养中显示秋水仙碱结合率降低，并且提取物具有与野生型相同的秋水仙碱结合特性。因此，抗性是由于通透性屏障所致。叙利亚仓鼠细胞系的天然抗性对秋水仙碱具有特异性，可能是由于特定的通透性屏障。此外，叙利亚仓鼠细胞系还显示有一个额外的秋水仙碱结合池。
