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裂殖酵母对秋水仙酰胺的敏感性：抗性突变体的通透性和解毒特性

Colcemid sensitivity of fission yeast: permeability and detoxification properties of resistant mutants.

作者信息

Lederberg S, Gourse R L, Sackett D L

出版信息

J Bacteriol. 1977 Jan;129(1):198-201. doi: 10.1128/jb.129.1.198-201.1977.

DOI:10.1128/jb.129.1.198-201.1977
PMID:830640
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC234915/
Abstract

Mutants of Schizosaccharomyces pombe, which were resistant to concentrations of colcemid inhibitory to the wild-type parent, were not diminished in their permeability to colcemid or colchicine. They also did not modify colcemid to a detectable derivative or to a product that was incapable of binding to brain tubulin. The resistance of these mutants was therefore via mechanisms different from those of permeability and detoxification known for animal cells.

摘要

粟酒裂殖酵母的突变体对秋水仙酰胺浓度具有抗性，而该浓度对野生型亲本具有抑制作用，这些突变体对秋水仙酰胺或秋水仙素的通透性并未降低。它们也没有将秋水仙酰胺修饰成可检测到的衍生物或无法与脑微管蛋白结合的产物。因此，这些突变体的抗性是通过与动物细胞已知的通透性和解毒机制不同的机制产生的。

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Colcemid sensitivity of fission yeast: permeability and detoxification properties of resistant mutants.裂殖酵母对秋水仙酰胺的敏感性：抗性突变体的通透性和解毒特性
J Bacteriol. 1977 Jan;129(1):198-201. doi: 10.1128/jb.129.1.198-201.1977.
2
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引用本文的文献

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Mol Gen Genet. 1980;180(1):231-4. doi: 10.1007/BF00267375.

本文引用的文献

1
AMINO ACID ACCUMULATION IN ETHIONINE-RESISTANT SACCHAROMYCES CEREVISIAE.抗乙硫氨酸酿酒酵母中的氨基酸积累
J Bacteriol. 1964 Jul;88(1):20-4. doi: 10.1128/jb.88.1.20-24.1964.
2
The mechanism of action of colchicine. Binding of colchincine-3H to cellular protein.秋水仙碱的作用机制。秋水仙碱-3H与细胞蛋白的结合。
J Cell Biol. 1967 Aug;34(2):525-33. doi: 10.1083/jcb.34.2.525.
3
Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. I. Evidence for a specific arginine-transporting system.酿酒酵母中氨基酸通透酶的多样性。I. 特定精氨酸转运系统的证据。
Biochim Biophys Acta. 1966 Oct 31;127(2):325-38. doi: 10.1016/0304-4165(66)90387-4.
4
The biochemical events of mitosis. I. Synthesis and properties of colchicine labeled with tritium in its acetyl moiety.有丝分裂的生化事件。I. 秋水仙碱乙酰基部分标记有氚的合成与性质。
Biochemistry. 1966 Jul;5(7):2463-8. doi: 10.1021/bi00871a042.
5
Colcemid sensitivity of fission yeast and the isolation of colcemid-resistant mutants.裂殖酵母对秋水仙酰胺的敏感性及秋水仙酰胺抗性突变体的分离
Science. 1970 Apr 24;168(3930):485-7. doi: 10.1126/science.168.3930.485.
6
Reduced permeability in CHO cells as a mechanism of resistance to colchicine.CHO细胞中通透性降低作为对秋水仙碱耐药的一种机制。
J Cell Physiol. 1974 Feb;83(1):103-16. doi: 10.1002/jcp.1040830114.
7
Colcemid inhibition of cell growth and the characterization of a colcemid-binding activity in Saccharomyces cerevisiae.秋水仙酰胺对酿酒酵母细胞生长的抑制作用及秋水仙酰胺结合活性的特性研究。
J Cell Biol. 1972 Nov;55(2):355-67. doi: 10.1083/jcb.55.2.355.
8
Metabolic transformation of colchicine, II. The metabolism of colchicine by mammalian liver microsomes.秋水仙碱的代谢转化，II. 秋水仙碱在哺乳动物肝脏微粒体中的代谢
Hoppe Seylers Z Physiol Chem. 1974 Nov;355(11):1391-9. doi: 10.1515/bchm2.1974.355.2.1391.
9
Adenylylstreptomycin, a product of streptomycin inactivated by E. coli carrying R factor.腺苷链霉素，一种由携带R因子的大肠杆菌使链霉素失活后产生的产物。
J Antibiot (Tokyo). 1968 Jan;21(1):81-2. doi: 10.7164/antibiotics.21.81.
10
Chloramphenicol-, dihydrostreptomycin-, and kanamycin-inactivating enzymes from multiple drug-resistant Escherichia coli carrying episome 'R'.
Nature. 1965 Dec 25;208(5017):1301-3. doi: 10.1038/2081301a0.