Duggan M A, Boras V F, Inoue M, McGregor S E
Department of Pathology, Foothills Hospital, Calgary, Alberta, Canada.
Am J Clin Pathol. 1991 Sep;96(3):318-25. doi: 10.1093/ajcp/96.3.318.
Because the sensitivities of individual hybridization techniques differ considerably, their role in accounting for the published frequencies of human papillomavirus (HPV) DNA in anal squamous cell carcinomas, ranging from 0 to 61%, must be investigated. With the use of biotinylated probes to HPV 6, 11, 16, 18, and 33, three hybridization techniques were performed on the same paraffin-embedded tissue blocks selected from 13 cases of anal squamous cell carcinoma. HPV DNA was detected in 0%, 62%, and 85% of cases with the use of in situ hybridization with horseradish peroxidase, in situ hybridization with alkaline phosphatase, and dot blot hybridization, respectively. By dot blot hybridization, 69% had HPV 16/6 and 15% had HPV 6/11. An HPV DNA frequency range of 0-85% in the same group of tumors with the use of three hybridization techniques indicates the influential role of the method on HPV DNA prevalences. HPV DNA was identified regardless of patient gender or type of squamous cell carcinoma. The presence of HPV 16 in 82% of the positive cases in supportive evidence of the carcinogenic role of the HPV in anal squamous cell carcinoma.
由于个体杂交技术的敏感性差异很大，因此必须研究它们在解释已发表的肛门鳞状细胞癌中人乳头瘤病毒（HPV）DNA频率（范围为0%至61%）方面的作用。使用针对HPV 6、11、16、18和33的生物素化探针，对从13例肛门鳞状细胞癌中选取的相同石蜡包埋组织块进行了三种杂交技术检测。分别使用辣根过氧化物酶原位杂交、碱性磷酸酶原位杂交和斑点印迹杂交，检测到HPV DNA的病例比例分别为0%、62%和85%。通过斑点印迹杂交，69%的病例检测到HPV 16/6，15%的病例检测到HPV 6/11。在同一组肿瘤中使用三种杂交技术检测到的HPV DNA频率范围为0%至85%，这表明检测方法对HPV DNA流行率有影响。无论患者性别或鳞状细胞癌类型如何，均检测到HPV DNA。82%的阳性病例中存在HPV 16，这支持了HPV在肛门鳞状细胞癌中的致癌作用。
