Li Jiu-Jun, Yu Zhi-Ling, Xue Xin-Dong
Department of Pediatrics, Second Hospital of China Medical University, Shenyang 110004, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2006 Feb;8(1):41-4.
To study the effect of captopril on the histopathology and bronchoalveolar lavage fluid (BALF) in neonatal rats exposed to hyperoxia.
Forty term neonatal Wistar rats were randomly assigned into Air control, Model, Normal saline control and Captopril-treated groups (n=10 each). The Air control group was exposed to air (FiO2=0.21). The remaining three groups were continuously exposed to hyperoxia (FiO2=0.90) . During exposure the Captopril-treated group received intragastric captopril (60 mg/kg daily) and the Normal saline control group was administered with normal saline. The Model group had no treatment. At the 14th and 21st days of exposure, the subjects were sacrificed. The lung coefficient and the protein contents and inflammatory cells in BALF were determined. The changes of lung histomorphology were observed.
The lung coefficient and the protein contents, the total number of cells and the percentage of neutrophils, lymphocytes and eosinophils in BAFL increased significantly in the Model and Normal saline control groups on the 14th and 21st days of exposure compared with those of the Air control group. Captopril treatment significantly reduced the lung coefficient and the protein contents, the total number of cells and the percentage of neutrophils and eosinophils in BALF. On the 14th day the lung coefficient decreased from 9.72 +/- 0.67 mg/g to 8.63 +/- 0.35 mg/g (P < 0.05); the protein contents in BALF from 0.619 +/- 0.023 g/L to 0.486 +/- 0.027 g/L (P < 0.05); and the total number of cells in BALF from (80.57 +/- 9.28)x10(4)/mL to (48.62 +/- 1.53)x10(4)/mL (P < 0.01) compared with the Model group. On the 21st day the lung coefficient decreased from 10.67 +/- 0.87 mg/g to 8.76 +/- 0.89 mg/g (P < 0.05); the protein contents in BALF from 0.978 +/- 0.012 g/L to 0.759 +/- 0.042 g/L (P < 0.05); and the total number of cells in BALF from (92.86 +/- 10.32) x10(4)/mL to (35.52 +/- 3.89) x10(4)/mL (P < 0.05) compared with the Model group. There were however significant differences in these results between the Captopril-treated and Air control groups. The histopathological examination demonstrated different degrees of alveolitis, broaden interstitium and reduced alveolar quantity in the Model and Normal saline control groups. The pathological changes were markedly alleviated after captopril treatment.
Captopril may have protective effects on lung injury induced by hyperoxia.
研究卡托普利对高氧暴露新生大鼠组织病理学及支气管肺泡灌洗液（BALF）的影响。
40只足月新生Wistar大鼠随机分为空气对照组、模型组、生理盐水对照组和卡托普利治疗组（每组n = 10）。空气对照组暴露于空气（FiO2 = 0.21）。其余三组持续暴露于高氧环境（FiO2 = 0.90）。暴露期间，卡托普利治疗组给予胃内灌注卡托普利（每日60 mg/kg），生理盐水对照组给予生理盐水，模型组不做处理。在暴露第14天和第21天处死动物，测定肺系数、BALF中蛋白质含量及炎症细胞数量，观察肺组织形态学变化。
与空气对照组相比，模型组和生理盐水对照组在暴露第14天和第21天时肺系数、BALF中蛋白质含量、细胞总数以及中性粒细胞、淋巴细胞和嗜酸性粒细胞百分比均显著增加。卡托普利治疗显著降低了肺系数、BALF中蛋白质含量、细胞总数以及中性粒细胞和嗜酸性粒细胞百分比。与模型组相比，第14天时肺系数从9.72±0.67 mg/g降至8.63±0.35 mg/g（P < 0.05）；BALF中蛋白质含量从0.619±0.023 g/L降至0.486±0.027 g/L（P < 0.05）；BALF中细胞总数从(80.57±9.28)×10(4)/mL降至(48.62±1.53)×10(4)/mL（P < 0.01）。第21天时肺系数从10.67±0.87 mg/g降至8.76±0.89 mg/g（P < 0.05）；BALF中蛋白质含量从0.978±0.012 g/L降至0.759±0.042 g/L（P < 0.05）；BALF中细胞总数从(92.86±10.32)×10(4)/mL降至(35.52±3.89)×10(4)/mL（P < 0.05）。然而，卡托普利治疗组与空气对照组之间这些结果存在显著差异。组织病理学检查显示，模型组和生理盐水对照组存在不同程度的肺泡炎、间质增宽和肺泡数量减少，卡托普利治疗后病理变化明显减轻。
卡托普利可能对高氧诱导的肺损伤具有保护作用。
