Maeda N, Seike M, Kume S, Takaku T, Shiga T
Department of Physiology, School of Medicine, Ehime University, Japan.
Biochim Biophys Acta. 1987 Nov 2;904(1):81-91. doi: 10.1016/0005-2736(87)90089-7.
The effect of fibrinogen and fibrinogen-derived products on the velocity of rouleau formation of human erythrocytes was quantitatively examined with a rheoscope combined with a video-camera, an image analyzer and a computer. (i) The velocity of rouleau formation by naturally occurring low-molecular-weight fibrinogen of 305 kDa and by desialylated fibrinogen was the same as that by native fibrinogen of 340 kDa. (ii) Concerning fibrinogen degradation products by plasmin, the velocity of rouleau formation decreased upon going from fibrinogen greater than fragment X greater than fragment Y (the ratio of molar concentration of fibrinogen, fragment X and fragment Y for giving a certain velocity of rouleau formation was approx. 1:2:5). The effect of fragments X and Y on the fibrinogen-induced rouleau formation was additive. (iii) Fragments D and E could not induce rouleau formation and did not affect the fibrinogen-, fragment X- and fragment Y-induced rouleau formation. (iv) Fibrinopeptides A and B and artificial tetrapeptides (Gly-Pro-Arg-Pro and Gly-His-Arg-Pro) did not affect the fibrinogen-induced rouleau formation. (v) The possible erythrocyte-binding site in fibrinogen molecule for leading to rouleaux was proposed to be in A alpha-chain (probably, around residues No. 207-303) near the terminal domain of the trinodular structure of fibrinogen.
使用结合了摄像机、图像分析仪和计算机的血流仪，定量检测了纤维蛋白原及其衍生产品对人红细胞缗钱状形成速度的影响。（i）305 kDa的天然低分子量纤维蛋白原和去唾液酸化纤维蛋白原的缗钱状形成速度与340 kDa的天然纤维蛋白原相同。（ii）关于纤溶酶产生的纤维蛋白原降解产物，从纤维蛋白原到大于X片段大于Y片段，缗钱状形成速度降低（为达到一定缗钱状形成速度，纤维蛋白原、X片段和Y片段的摩尔浓度比约为1:2:5）。X片段和Y片段对纤维蛋白原诱导的缗钱状形成的影响是相加的。（iii）D片段和E片段不能诱导缗钱状形成，也不影响纤维蛋白原、X片段和Y片段诱导的缗钱状形成。（iv）纤维蛋白肽A和B以及人工四肽（甘氨酸-脯氨酸-精氨酸-脯氨酸和甘氨酸-组氨酸-精氨酸-脯氨酸）不影响纤维蛋白原诱导的缗钱状形成。（v）纤维蛋白原分子中可能导致缗钱状形成的红细胞结合位点被认为位于纤维蛋白原三结节结构末端结构域附近的Aα链（可能在第207 - 303位残基附近）。
