van der Grond J, Van Pelt A M, van Echteld C J, Dijkstra G, Grootegoed J A, de Rooij D G, Mali W P
Department of Radiodiagnosis, University of Utrecht, The Netherlands.
Biol Reprod. 1991 Jul;45(1):122-7. doi: 10.1095/biolreprod45.1.122.
Testes of vitamin A-deficient Wistar rats before and after vitamin A replacement, of rats irradiated in utero, and of control rats were investigated by in vivo 31P magnetic resonance (MR) spectroscopy. The testicular phosphomonoester/ATP (PM/ATP) ratio ranged from 0.79 +/- 0.05 for testes that contained only interstitial tissue and Sertoli cells to 1.64 +/- 0.04 for testes in which spermatocytes were the most advanced cell types present. When new generations of spermatids entered the seminiferous epithelium, this ratio decreased. The testicular phosphodiester/ATP (PD/ATP) ratio amounted to 0.16 +/- 0.06 for testes in which Sertoli cells, spermatogonia, or spermatocytes were the most advanced cell type present. When new generations of spermatids entered the seminiferous epithelium, the PD/ATP ratio rapidly increased and finally reached a value of 0.71 +/- 0.06 for fully developed testes. Taken together, specific patterns of the PM/ATP ratio, the PD/ATP ratio, and pH were obtained that were correlated to the presence of spermatogonia, spermatocytes, round spermatids, and elongated spermatids or to the absence of spermatogenic cells. Hence, a good impression of the status of the seminiferous epithelium in the rat can be obtained by in vivo 31P MR spectroscopy.
通过体内³¹P磁共振（MR）波谱法，对维生素A缺乏的Wistar大鼠在维生素A替代前后、子宫内受照射大鼠以及对照大鼠的睾丸进行了研究。睾丸磷酸单酯/三磷酸腺苷（PM/ATP）比值范围为：仅含间质组织和支持细胞的睾丸为0.79±0.05，以精母细胞为最成熟细胞类型的睾丸为1.64±0.04。当新一代精子细胞进入生精上皮时，该比值下降。睾丸磷酸二酯/三磷酸腺苷（PD/ATP）比值在以支持细胞、精原细胞或精母细胞为最成熟细胞类型的睾丸中为0.16±0.06。当新一代精子细胞进入生精上皮时，PD/ATP比值迅速升高，最终在完全发育的睾丸中达到0.71±0.06的值。综合来看，获得了与精原细胞、精母细胞、圆形精子细胞和长形精子细胞的存在或生精细胞的缺失相关的PM/ATP比值、PD/ATP比值和pH的特定模式。因此，通过体内³¹P MR波谱法可以很好地了解大鼠生精上皮的状态。
