Rius R A, Streaty R A, Peng Loh Y, Klee W A
Section on Cellular Neurobiology, National Institute of Child Health and Human Development, Bethesda, MD 20892.
FEBS Lett. 1991 Aug 19;288(1-2):51-4. doi: 10.1016/0014-5793(91)81001-o.
Changes in the relative abundance of the G protein alpha subunits were observed during early mouse development Gs alpha was almost exclusively present as a large form (Gs-1) in prenatal brain. Postnatally with a substantial increase in Gpp[NH]p stimulated adenylyl cyclase activity, the small form (Gs.s) increased in amount while Gs-1 decreased. These results suggest that the Gs-s may be the more effective cyclase activator and that changes in alternative splicing are developmentally regulated. Gi1 and Go appeared before birth whereas Gi2 developed postnatally. Opiate stimulation of GTPase and inhibition of adenylyl cyclase were fully expressed prenatally.
在小鼠早期发育过程中观察到G蛋白α亚基相对丰度的变化。Gsα在产前大脑中几乎仅以一种大的形式（Gs-1）存在。出生后，随着Gpp[NH]p刺激的腺苷酸环化酶活性大幅增加，小的形式（Gs.s）数量增加，而Gs-1减少。这些结果表明Gs.s可能是更有效的环化酶激活剂，并且可变剪接的变化受发育调控。Gi1和Go在出生前出现，而Gi2在出生后发育。阿片类物质对GTP酶的刺激和对腺苷酸环化酶的抑制在产前就已充分表现出来。
