Juniper S, Abbott L K
School of Earth and Geographical Sciences, Faculty of Natural and Agricultural Sciences, The University of Western Australia, Nedlands, WA, 6009, Australia.
Mycorrhiza. 2006 Jul;16(5):371-9. doi: 10.1007/s00572-006-0046-9. Epub 2006 Mar 9.
Colonisation of plant roots by some arbuscular mycorrhizal (AM) fungi is reduced in the presence of sodium chloride (NaCl), probably due to a direct effect of NaCl on the fungi. However, there appear to be differences between the fungi in their ability to colonise plants in the presence of NaCl. This experiment tested the hypothesis that propagules of different isolates and species of AM fungi from saline and nonsaline soils would differ in their ability to germinate and grow in the presence of NaCl in the soil solution. Spores or pieces of root colonised by a range of AM fungi were incubated between filters buried in soil to which NaCl had been added at concentrations of 0, 150 or 300 mM in the soil solution. At regular intervals, filters were removed from the soil and both the percentage of propagules which had germinated and the length of proliferating hyphae were determined. Germination of spores of AM fungi studied was delayed in the presence of NaCl, but the fungi differed in the extent to which germination was inhibited. Two isolates of Scutellospora calospora reached maximum germination in 300 mM NaCl, but neither of two isolates of Acaulospora laevis germinated in the presence of NaCl. Germination of spores of the other fungi, including some isolated from saline soil, fell between these extremes. For some fungi, the specific rate of hyphal extension was reduced by NaCl. For others, the specific rate of growth was similar in the presence of NaCl to that in the control treatment, but overall production of hyphae was reduced in the NaCl treatments because germination was reduced.
在氯化钠（NaCl）存在的情况下，一些丛枝菌根（AM）真菌对植物根系的定殖会减少，这可能是由于NaCl对真菌的直接影响。然而，不同真菌在NaCl存在时定殖植物的能力似乎存在差异。本实验检验了以下假设：来自盐渍土和非盐渍土的不同分离株和物种的AM真菌繁殖体在土壤溶液中存在NaCl的情况下，其发芽和生长能力会有所不同。将一系列AM真菌定殖的孢子或根段置于埋于添加了NaCl的土壤中的滤膜之间进行培养，土壤溶液中NaCl的浓度分别为0、150或300 mM。定期从土壤中取出滤膜，测定已发芽繁殖体的百分比和增殖菌丝的长度。所研究的AM真菌孢子的发芽在NaCl存在时延迟，但不同真菌的发芽受抑制程度不同。两种厚壁孢子囊霉分离株在300 mM NaCl中达到最大发芽率，但光滑无梗囊霉的两种分离株在NaCl存在时均未发芽。其他真菌（包括一些从盐渍土分离的真菌）的孢子发芽情况介于这两个极端之间。对于一些真菌，NaCl降低了菌丝延伸的特定速率。对于其他真菌，在NaCl存在时的特定生长速率与对照处理相似，但由于发芽率降低，NaCl处理中菌丝的总体产量减少。
