van der Aa Marieke A E M, Mastrobattista Enrico, Oosting Ronald S, Hennink Wim E, Koning Gerben A, Crommelin Daan J A
Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, 3508 TB, Utrecht, The Netherlands.
Pharm Res. 2006 Mar;23(3):447-59. doi: 10.1007/s11095-005-9445-4. Epub 2006 Mar 15.
One of the limiting steps in the efficiency of nonviral gene delivery is transport of genetic material across the nuclear membrane. Trafficking of nuclear proteins from the cytoplasm into the nucleus occurs via the nuclear pore complex and is mediated by nuclear localization signals and their nuclear receptors. Several strategies employing this transport mechanism have been designed and explored to improve nonviral gene delivery. In this article, we review the mechanism of nuclear import through the nuclear pore complex and the strategies used to facilitate nuclear import of exogenous DNA and improve gene expression.
非病毒基因递送效率的限制步骤之一是遗传物质穿过核膜的运输。核蛋白从细胞质进入细胞核的运输是通过核孔复合体进行的，并由核定位信号及其核受体介导。已经设计并探索了几种利用这种运输机制来改善非病毒基因递送的策略。在本文中，我们综述了通过核孔复合体进行核输入的机制以及用于促进外源DNA核输入并改善基因表达的策略。
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