Sayarlioglu Hayriye, Dogan Ekrem, Erkoc Reha, Ozbek Hanefi, Bayram Irfan, Sayarlioglu Mehmet, Sekeroglu Ramazan, Bozkurt Hamza
Department of Internal Medicine, Division of Nephrology Yuzuncu Yil University, Medical Faculty, Van, Turkey.
Ren Fail. 2006;28(1):69-75. doi: 10.1080/08860220500461286.
Peritoneal dialysis (PD) is a treatment modality for patients with renal failure. Peritoneal fibrosis is one of the most serious complications after long-term continuous ambulatory peritoneal dialysis (CAPD). Histological studies in both humans and animals show that chronic peritoneal dialysis results in fibrosis of the peritoneal membrane. In our study, we investigated the effect of colchicine on peritoneal alterations induced by hypertonic PD solution in rats. Sprague-Dawley rats intraperitoneally received saline (control group) once daily, for 28 days, or 3.86% glucose (PDF group), or 3.86% glucose plus colchicine (colchicine group). Animals from each group were sacrificed after 28 days with anesthetized ketamine (60 mg/kg BW). For the PD fluid assessment, 1 h before the sacrifice of animals, 10 mL PD fluid of 2.27% glucose was given, and this fluid was obtained after the sacrifice. The levels of transforming endothelial growth factor beta (TGF-beta), tumor necrosis factor alpha (TNF-alpha) and albumin were investigated both in the peritoneal dialysate and blood, and the levels of malondialdehyde (MDA) were investigated only in peritoneal dialysate. The peritoneal membrane was evaluated histologically by light microscopy. When groups were compared in terms of body weight change, the colchicine group significantly lost weight compared to controls and PDF group (-4.7% + 4.5, 3.5% +/- 7.2, 3.0% +/- 1.3, respectively, p = 0.018). Also, the blood albumin level was significantly lower for these in the colchicine group compared to those in the PDF group (2.7 +/- 0.35 versus 3.2 +/- 0.3 g/dL, respectively, p = 0.048). The blood TGF-beta level was significantly lower in the control group, and no difference was observed between the PDF and colchicine groups (294.4 +/- 67.5 versus 787.4 +/- 237.4 versus 615.3 +/- 235.1 pg/mL, respectively, p = 0.004). The mesothelial thickness found in groups was as follows: control group 102 +/- 18.9 microm, PDF group 128.33 +/- 33.1 microm, colchicine group 117 +/- 35.6 microm (p = 0.34). In conclusion, a rat model for peritoneal dialysis associated peritoneal derangement without fibrosis could be induced. Colchicine could not prevent peritoneal derangement in this model.
腹膜透析（PD）是肾衰竭患者的一种治疗方式。腹膜纤维化是长期持续性非卧床腹膜透析（CAPD）后最严重的并发症之一。人体和动物的组织学研究表明，慢性腹膜透析会导致腹膜纤维化。在我们的研究中，我们调查了秋水仙碱对高渗腹膜透析液诱导的大鼠腹膜改变的影响。将Sprague-Dawley大鼠分为三组，分别每天腹腔注射一次生理盐水（对照组），持续28天；或注射3.86%葡萄糖（PDF组）；或注射3.86%葡萄糖加秋水仙碱（秋水仙碱组）。28天后，用氯胺酮（60mg/kg体重）麻醉处死每组动物。在处死动物前1小时，给予10mL 2.27%葡萄糖的腹膜透析液，处死动物后收集该透析液。检测腹膜透析液和血液中转化生长因子β（TGF-β）、肿瘤坏死因子α（TNF-α）和白蛋白的水平，仅在腹膜透析液中检测丙二醛（MDA）的水平。通过光学显微镜对腹膜进行组织学评估。比较各组体重变化时，秋水仙碱组与对照组和PDF组相比体重显著下降（分别为-4.7%±4.5、3.5%±7.2、3.0%±1.3，p=0.018）。此外，秋水仙碱组的血白蛋白水平显著低于PDF组（分别为2.7±0.35与3.2±0.3g/dL，p=0.048）。对照组的血TGF-β水平显著较低，PDF组和秋水仙碱组之间未观察到差异（分别为294.4±67.5、787.4±237.4、615.3±235.1pg/mL，p=0.004）。各组间间皮厚度如下：对照组102±18.9μm，PDF组128.33±33.1μm，秋水仙碱组117±35.6μm（p=0.34）。总之，可以诱导出一种无纤维化的腹膜透析相关腹膜紊乱的大鼠模型。在该模型中，秋水仙碱不能预防腹膜紊乱。
