Kekarainen Tuija, Sibila Marina, Segalés Joaquim
Centre de Recerca en Sanitat Animal (CReSA), Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Departament de Sanitat i d'Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
J Gen Virol. 2006 Apr;87(Pt 4):833-837. doi: 10.1099/vir.0.81586-0.
The present study was designed to investigate the prevalence of swine Torque teno virus (TTV) in post-weaning multisystemic wasting syndrome (PMWS)-affected and non-affected Spanish swine. Nested PCR (nPCR) assays to detect two distinct TTV genogroups were applied. A significantly higher prevalence of TTV infection was found in sera from PMWS-affected animals (97 %) than in sera from non-PMWS-affected animals (78 %). Whilst PMWS-affected pigs (91 %) were more likely to be infected with TTV from genogroup 2 than non-PMWS-affected swine (72 %), no such difference was observed with genogroup 1. Nucleotide sequences of nPCR products were 91-99 % identical between strains within a genogroup. In contrast, inter-genogroup sequence identities were 49-58 %. Phylogenetic analyses demonstrated that genogroups form different clusters without association with PMWS or porcine circovirus type 2 infection status of the animals. These results indicate a high prevalence of both swine TTV genogroups in Spain, being present more frequently in PMWS-affected animals than in non-PMWS-affected animals.
本研究旨在调查断奶后多系统消耗综合征（PMWS）感染和未感染的西班牙猪中猪圆环病毒（TTV）的流行情况。应用巢式PCR（nPCR）检测两种不同的TTV基因组。结果发现，PMWS感染动物血清中TTV感染率（97%）显著高于未感染PMWS动物血清（78%）。虽然感染PMWS的猪（91%）比未感染PMWS的猪（72%）更易感染基因组2的TTV，但基因组1未观察到这种差异。基因组内菌株间nPCR产物的核苷酸序列同一性为91%-99%。相比之下，基因组间的序列同一性为49%-58%。系统发育分析表明，基因组形成不同的簇，与动物的PMWS或2型猪圆环病毒感染状态无关。这些结果表明，西班牙两种猪TTV基因组的流行率都很高，在感染PMWS的动物中比未感染PMWS的动物中更常见。
