Segalés Joaquim, Martínez-Guinó Laura, Cortey Martí, Navarro Nuria, Huerta Eva, Sibila Marina, Pujols Joan, Kekarainen Tuija
Centre de Recerca en Sanitat Animal, UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Vet Microbiol. 2009 Mar 2;134(3-4):199-207. doi: 10.1016/j.vetmic.2008.08.002. Epub 2008 Aug 20.
A retrospective study to detect evidence of swine Torque teno virus (TTV) genogroups 1 and 2 infection in sera of pigs from the Spanish livestock between years 1985 and 2005 was carried out by means of PCR. Also, the molecular evolution of TTV genogroups 1 and 2 during the 20-year period studied using a 250-base sequence of the non-coding region of the viral genome was assessed. Both TTV genogroup genomes were found in pig sera from the first year of study. Taking into account the whole study period, 113 out of 162 animals (69.8%) were infected with one or the other genogroup, while 38 out of 162 pigs (23.5%) were co-infected with both genogroups. Moreover, TTV genogroup 2 (90 out of 162, 55.6%) was significantly more prevalent than genogroup 1 (54 out of 162, 33.3%). The non-coding region of swine TTV genome sequenced showed its adequacy as a molecular marker in swine TTV. This study represents the earliest evidence of TTV infection in pigs to date, 14 years before the initial description of this virus. Moreover, this is also the earliest evidence of TTV infection in any species.
通过聚合酶链反应（PCR）对1985年至2005年间西班牙家畜猪血清中的猪Torque teno病毒（TTV）基因组1型和2型感染证据进行了一项回顾性研究。此外，还评估了在研究的20年期间，使用病毒基因组非编码区的250个碱基序列对TTV基因组1型和2型的分子进化情况。在研究的第一年猪血清中就发现了两种TTV基因组。考虑到整个研究期间，162只动物中有113只（69.8%）感染了其中一个基因组，而162头猪中有38头（23.5%）同时感染了两个基因组。此外，TTV基因组2型（162只中有90只，55.6%）的流行率显著高于基因组1型（162只中有54只，33.3%）。测序的猪TTV基因组非编码区显示其作为猪TTV分子标记的适用性。这项研究是迄今为止猪TTV感染的最早证据，比该病毒最初描述早14年。此外，这也是TTV在任何物种中感染的最早证据。
